Hematology, Oncology, and Transfusion Medicine Center, Vilnius University Hospital Santariskiu Clinics, Santariskiu 2, Vilnius, Lithuania.
Cytometry B Clin Cytom. 2011 Sep;80(5):318-23. doi: 10.1002/cyto.b.20601. Epub 2011 Jun 1.
Both normal and malignant plasma cell (PC) populations can be identified using modern flow cytometry (FC) technique in multiple myeloma (MM) patients. Expression of CD19 and CD56 markers is heterogenous on bone marrow PCs of healthy individuals. Little is known about immunophenotypically aberrant (CD19-/CD56+) PCs subpopulation of healthy people.
Using six color FC, we analyzed PCs in BM samples of 11 healthy donors (HD) and compared their immunophenotypic properties with clonal PC populations from MM patients.
Both immunophenotypically normal (CD19+/CD56-) and aberrant (CD19-/CD56+) PC populations could be detected in 10 of 11 HDs' BM samples and constituted the median of 60.3% (37.3-72.3) and 9.6% (0-35.7) of BM PCs, respectively. CD19, CD56, CD38, CD45, and CD20 marker expression characteristics were of little value discriminating clonal PCs of MM patients from immunophenotypically aberrant PCs of healthy donors.
Our findings suggest that aberrant immunophenotype is common in BM PCs of healthy people. Improvements in FC methodology to separate normal and malignant PCs remain an open area for future investigations.
在多发性骨髓瘤（MM）患者中，现代流式细胞术（FC）技术可识别正常和恶性浆细胞（PC）群体。健康个体骨髓 PC 上的 CD19 和 CD56 标志物表达具有异质性。对于健康人群中免疫表型异常（CD19-/CD56+）PC 亚群知之甚少。
使用六色 FC，我们分析了 11 名健康供体（HD）的 BM 样本中的 PC，并将其免疫表型特性与 MM 患者的克隆性 PC 群体进行比较。
在 11 名 HD 的 BM 样本中均可以检测到免疫表型正常（CD19+/CD56-）和异常（CD19-/CD56+）PC 群体，分别构成 BM PC 的中位数的 60.3%（37.3-72.3）和 9.6%（0-35.7）。CD19、CD56、CD38、CD45 和 CD20 标志物表达特征对于区分 MM 患者的克隆性 PCs 和健康供体的免疫表型异常 PCs 几乎没有价值。
我们的研究结果表明，异常免疫表型在健康人群的 BM PC 中很常见。改进 FC 方法以分离正常和恶性 PC 仍然是未来研究的一个未解决的领域。
