Ito H, Yamamoto H, Kimura Y, Kambe H, Okochi T, Kishimoto S
Third Department of Internal Medicine, Osaka University School of Medicine, Japan.
J Chromatogr. 1990 Apr 6;526(2):397-406. doi: 10.1016/s0378-4347(00)82523-2.
Human plasma gelsolin was specifically eluted from a Cibacron Blue F3GA column with 1 mM adenosine, guanosine, cytidine and uridine di- and triphosphates, except for cytidine 5'-diphosphate. Inorganic polyphosphates also eluted gelsolin, but neither nicotinamide adenine dinucleotide nor nicotinamide adenine dinucleotide phosphate did so. The results suggest that a terminal pyrophosphate structure might be essential for the specific elution. After elution with 1 mM adenosine 5'-triphosphate, additional gelsolin was eluted by washing the column with a high salt concentration. This indicates that human plasma gelsolin may bind to Cibacron Blue F3GA in at least two different ways.
人血浆凝溶胶蛋白可通过用1 mM腺苷、鸟苷、胞苷和尿苷二磷酸及三磷酸从Cibacron Blue F3GA柱上特异性洗脱下来，但胞苷5'-二磷酸除外。无机多磷酸盐也能洗脱凝溶胶蛋白，但烟酰胺腺嘌呤二核苷酸和烟酰胺腺嘌呤二核苷酸磷酸均不能。结果表明，末端焦磷酸结构可能是特异性洗脱所必需的。用1 mM腺苷5'-三磷酸洗脱后，通过用高盐浓度冲洗柱子可进一步洗脱凝溶胶蛋白。这表明人血浆凝溶胶蛋白可能至少以两种不同方式与Cibacron Blue F3GA结合。
