Human plasma gelsolin binds adenosine triphosphate.
作者信息
Yamamoto H, Ito H, Nakamura H, Hayashi E, Kishimoto S, Hashimoto T, Tagawa K
机构信息
Third Department of Internal Medicine, Osaka University School of Medicine.
出版信息
J Biochem. 1990 Oct;108(4):505-6. doi: 10.1093/oxfordjournals.jbchem.a123229.
Abstract
Binding studies of human plasma gelsolin with ATP were done by equilibrium dialysis. Analysis of the binding data showed that plasma gelsolin had one class of ATP binding site with Kd = 2.8 x 10(-7) M, which saturated at an ATP/gelsolin ratio of 0.6. The bioluminescent assay for ATP with luciferin and firefly luciferase confirmed that the protein contained a nucleotide as ATP.
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