Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682 041, India.
Int J Biol Macromol. 2011 Oct 1;49(3):247-54. doi: 10.1016/j.ijbiomac.2011.04.005. Epub 2011 May 27.
Sodium alginate (SA)/poly (vinyl alcohol) (PVA) fibrous mats were prepared by electrospinning technique. ZnO nanoparticles of size ∼160nm was synthesized and characterized by UV spectroscopy, dynamic light scattering (DLS), XRD and infrared spectroscopy (IR). SA/PVA electrospinning was further carried out with ZnO with different concentrations (0.5, 1, 2 and 5%) to get SA/PVA/ZnO composite nanofibers. The prepared composite nanofibers were characterized using FT-IR, XRD, TGA and SEM studies. Cytotoxicity studies performed to examine the cytocompatibility of bare and composite SA/PVA fibers indicate that those with 0.5 and 1% ZnO concentrations are less toxic where as those with higher concentrations of ZnO is toxic in nature. Cell adhesion potential of this mats were further proved by studying with L929 cells for different time intervals. Antibacterial activity of SA/PVA/ZnO mats were examined with two different bacteria strains; Staphylococcus aureus and Escherichia coli, and found that SA/PVA/ZnO mats shows antibacterial activity due to the presence of ZnO. Our results suggest that this could be an ideal biomaterial for wound dressing applications once the optimal concentration of ZnO which will give least toxicity while providing maximum antibacterial activity is identified.f.
海藻酸钠（SA）/聚乙烯醇（PVA）纤维垫是通过静电纺丝技术制备的。合成了尺寸约为 160nm 的 ZnO 纳米粒子，并通过紫外光谱、动态光散射（DLS）、XRD 和红外光谱（IR）进行了表征。进一步用不同浓度（0.5、1、2 和 5%）的 ZnO 进行 SA/PVA 静电纺丝，得到 SA/PVA/ZnO 复合纳米纤维。使用 FT-IR、XRD、TGA 和 SEM 研究对制备的复合纳米纤维进行了表征。进行细胞毒性研究以检查裸纤维和复合 SA/PVA 纤维的细胞相容性，结果表明，浓度为 0.5%和 1%的 ZnO 的纤维毒性较小，而浓度较高的 ZnO 纤维具有毒性。通过研究不同时间间隔的 L929 细胞进一步证明了这些垫的细胞粘附潜力。用两种不同的细菌菌株（金黄色葡萄球菌和大肠杆菌）检查了 SA/PVA/ZnO 垫的抗菌活性，发现由于 ZnO 的存在，SA/PVA/ZnO 垫显示出抗菌活性。我们的结果表明，一旦确定了既能提供最大抗菌活性又能使毒性最小的 ZnO 的最佳浓度，它就可能成为一种理想的用于伤口敷贴的生物材料。
