Safari Parva, Rahimabadi Eshagh Zakipour, Vaezi Mohammad Reza, Behnamghader Aliasghar, Tahergorabi Reza
Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, 1144, Iran.
Research Department of Nanotechnology and Advanced Materials, , Materials and Energy Research Center, Karaj, Iran.
Sci Rep. 2025 May 13;15(1):16567. doi: 10.1038/s41598-025-01669-w.
This paper studied the possibility of fabricating a nano-composite based on chitosan incorporated with ZnO-NPs as a promising textile for wound dressing purposes. The nanofiber mat was obtained from dispersions of ZnO-NPs in chitosan-based solution blended with PVA (Cs/PVA/ZnO-NPs scaffold). The extracted chitosan was characterized using FTIR, FE-SEM, XRD, and TGA analysis. The electrospinning optimization process was successfully done for Cs and PVA mixture and a good combination of polymers, solvent, and the ratios developed through an optimization process (10wt.% PVA and 1wt.% CS in AcAcetic 80%). The nanofibers had an average diameter below 200 nm, while the incorporation of ZnO-NPs decreased their average diameter below 150 nm. FTIR, FE-SEM, XRD analysis were used to evaluate the scaffold structure. The FE-SEM analysis proved the smooth and bead-free morphology of the fibers. Elemental analysis of the mat revealed a good distribution of ZnO-NPs along nanofibers. Cell culture studies with L929 mouse fibroblast cells revealed good viability of the cell on the Cs/PVA/ZnO-NPs scaffold. The nanoparticles improved capability of the mat for growth inhibition rate of bacterial colonies and also its wettability. The results also showed the nontoxicity of CS/PVA/ZnO-NPs composite and its considerable potential for future application in wound dressing.
本文研究了制备一种基于壳聚糖并掺入氧化锌纳米颗粒（ZnO-NPs）的纳米复合材料作为有前景的伤口敷料纺织品的可能性。纳米纤维垫是由ZnO-NPs分散在与聚乙烯醇（PVA）混合的壳聚糖基溶液中获得的（壳聚糖/聚乙烯醇/ZnO-NPs支架）。使用傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FE-SEM）、X射线衍射（XRD）和热重分析（TGA）对提取的壳聚糖进行了表征。通过优化过程成功完成了壳聚糖和PVA混合物的静电纺丝优化，确定了聚合物、溶剂及其比例的良好组合（在80%的醋酸中含10wt.%的PVA和1wt.%的壳聚糖）。纳米纤维的平均直径低于200纳米，而掺入ZnO-NPs后其平均直径降至150纳米以下。使用FTIR、FE-SEM、XRD分析来评估支架结构。FE-SEM分析证明了纤维具有光滑且无珠的形态。对该垫子的元素分析表明ZnO-NPs沿纳米纤维分布良好。用L929小鼠成纤维细胞进行的细胞培养研究表明，细胞在壳聚糖/聚乙烯醇/ZnO-NPs支架上具有良好的活力。纳米颗粒提高了垫子对细菌菌落的生长抑制率及其润湿性。结果还表明壳聚糖/聚乙烯醇/ZnO-NPs复合材料无毒，在伤口敷料方面具有相当大的未来应用潜力。
