Peiper S C, Myers J L, Broussard E E, Sixbey J W
Department of Pathology, University of Alabama, Birmingham 35233.
Arch Pathol Lab Med. 1990 Jul;114(7):711-4.
We used oligonucleotide primers designed from DNA sequences unique to the long internal direct repeated region of Epstein-Barr virus (EBV) to enzymatically amplify this segment of the EBV genome in formalin-fixed, paraffin-embedded tissues. The products amplified from EBV templates were detected by hybridization with a labeled probe specific for this highly conserved, reiterated region. Epstein-Barr virus-related sequences were detected in the spleen of a patient with infectious mononucleosis, in lung and lymph node specimens from a patient with pulmonary manifestations of infectious mononucleosis, in various tissues from seven immunosuppressed organ transplant recipients with immunoproliferative disorders, and in small biopsy specimens from a patient with nasopharyngeal carcinoma. No viral sequences were detected in 20 histologically normal spleens or 10 lymph nodes. Polymerase chain reaction technology provides an effective means for documenting EBV infection in archival tissues. This approach should facilitate the diagnosis of posttransplant lymphoproliferative disorders and difficult cases of infectious mononucleosis and nasopharyngeal carcinoma.
我们使用了根据爱泼斯坦-巴尔病毒（EBV）长内部直接重复区域特有的DNA序列设计的寡核苷酸引物，以酶促扩增福尔马林固定、石蜡包埋组织中EBV基因组的这一片段。通过与针对这个高度保守的重复区域的标记探针杂交来检测从EBV模板扩增出的产物。在一名传染性单核细胞增多症患者的脾脏中、在一名有传染性单核细胞增多症肺部表现患者的肺和淋巴结标本中、在七名患有免疫增殖性疾病的免疫抑制器官移植受者的各种组织中以及在一名鼻咽癌患者的小活检标本中检测到了EBV相关序列。在20个组织学正常的脾脏或10个淋巴结中未检测到病毒序列。聚合酶链反应技术为记录存档组织中的EBV感染提供了一种有效方法。这种方法应有助于诊断移植后淋巴增殖性疾病以及传染性单核细胞增多症和鼻咽癌的疑难病例。
