Snijders P J, Schulten E A, Mullink H, ten Kate R W, Jiwa M, van der Waal I, Meijer C J, Walboomers J M
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Am J Pathol. 1990 Sep;137(3):659-66.
The presence of human papillomavirus (HPV) and Epstein-Barr virus (EBV) was analyzed in 21 oral biopsy specimens of HIV-infected patients using the polymerase chain reaction (PCR) method. Biopsies were categorized as hairy leukoplakia (HL) (n = 12), candidiasis (n = 3), oral warts (n = 2), and clinically normal epithelium (n = 4). For HPV detection a modified general primer-mediated PCR method (GP-PCR), which detects a broad spectrum of HPV genotypes at sub-picogram levels, was used. Human papillomavirus DNA was only found in two oral warts and was identified as HPV type 32. Epstein-Barr virus DNA was detected in 16 biopsy specimens, including the 12 HLs, 2 cases of candidiasis, and 2 samples of normal epithelium. Epstein-Barr virus positivity in HL could be confirmed by Southern blot analysis and DNA in situ hybridization using biotinylated DNA probes (bio-DISH). Epstein-Barr virus bio-DISH was also positive in one sample of normal epithelium from a patient with HL. The results indicate that HL is strongly associated with EBV and not with any of the common HPV types that react with general HPV primers in the PCR. However the detection of EBV in normal oral epithelium by PCR and bio-DISH suggests that the presence of this virus is not exclusively related to HL.
采用聚合酶链反应（PCR）方法，对21例HIV感染患者的口腔活检标本进行了人乳头瘤病毒（HPV）和爱泼斯坦-巴尔病毒（EBV）检测。活检标本分为毛状白斑（HL）（n = 12）、念珠菌病（n = 3）、口腔疣（n = 2）和临床正常上皮组织（n = 4）。HPV检测采用改良的通用引物介导的PCR方法（GP-PCR），该方法能在亚皮克级水平检测多种HPV基因型。仅在2例口腔疣中发现了人乳头瘤病毒DNA，鉴定为HPV 32型。在16例活检标本中检测到了爱泼斯坦-巴尔病毒DNA，包括12例HL、2例念珠菌病和2例正常上皮组织样本。HL中的爱泼斯坦-巴尔病毒阳性可通过Southern印迹分析和使用生物素化DNA探针的DNA原位杂交（bio-DISH）来确认。HL患者的1例正常上皮组织样本中，爱泼斯坦-巴尔病毒bio-DISH也呈阳性。结果表明，HL与EBV密切相关，与PCR中能与通用HPV引物反应的任何常见HPV类型均无关。然而，通过PCR和bio-DISH在正常口腔上皮组织中检测到EBV，提示该病毒的存在并非仅与HL相关。
