Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, Goiás State, Brazil.
Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
J Med Microbiol. 2011 Oct;60(Pt 10):1455-1459. doi: 10.1099/jmm.0.031450-0. Epub 2011 Jun 2.
Pneumococcal nasopharyngeal carriage isolates recovered from Brazilian children attending day-care centres in 2005 were assessed for serotype, genotype and penicillin susceptibility phenotype. As 124 of the 253 isolates (49 %) were characterized previously with respect to serotype and penicillin susceptibility, the primary objectives were to examine clonal associations and penicillin susceptibility within major serotypes and to assess the suitability of conventional multiplex PCR for deducing carriage serotypes within this population. Using a combination of PCR-based serotyping and the Quellung reaction, serotypes were identified for 81 % (205/253) of the isolates, with serogroups or types 14, 6, 23F, 19F and 18 being predominant. Included within the 205 isolates successfully serotyped by PCR were 28 isolates that had become non-viable. Forty-eight isolates were non-typable using both the PCR method and the Quellung reaction. Penicillin non-susceptibility was observed within 16 of the 18 multilocus sequence types detected. Thus, this study provides further evidence from a diverse collection of pneumococcal clones that PCR-based serotype deduction is useful for providing supportive evidence for pneumococcal conjugate vaccine implementation.
2005 年，从巴西日托中心的儿童鼻咽部分离出的肺炎球菌携带株被评估了血清型、基因型和青霉素敏感性表型。由于 253 株分离株中有 124 株（49%）之前已经对血清型和青霉素敏感性进行了特征描述，因此主要目的是检查主要血清型中的克隆相关性和青霉素敏感性，并评估常规多重 PCR 在此人群中推断携带血清型的适用性。通过聚合酶链反应（PCR）基础血清分型和胶乳反应相结合的方法，对 253 株分离株中的 81%（205/253）进行了血清型鉴定，血清型 14、6、23F、19F 和 18 血清型为主导。在通过 PCR 成功定型的 205 株分离株中，有 28 株分离株失去了活力。48 株分离株使用 PCR 方法和胶乳反应均无法定型。在检测到的 18 种多位点序列型中，有 16 种对青霉素不敏感。因此，本研究从多样化的肺炎球菌克隆群中提供了进一步的证据，表明基于 PCR 的血清型推断对于支持肺炎球菌结合疫苗的实施是有用的。
