Cardona-Felix Cesar S, Lara-Gonzalez Samuel, Brieba Luis G
Grupo de Bioquímica Estructural, Laboratorio Nacional de Genomica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Km 9.6 Libramiento Norte, Carretera Irapuato-León, 36821 Irapuato, Guanajuato, México.
Acta Crystallogr D Biol Crystallogr. 2011 Jun;67(Pt 6):497-505. doi: 10.1107/S0907444911010547. Epub 2011 May 12.
Proliferating cellular nuclear antigen (PCNA) is a toroidal-shaped protein that is involved in cell-cycle control, DNA replication and DNA repair. Parasitic protozoa are early-diverged eukaryotes that are responsible for neglected diseases. In this work, a PCNA from a parasitic protozoon was identified, cloned and biochemically characterized and its crystal structure was determined. Structural and biochemical studies demonstrate that PCNA from Entamoeba histolytica assembles as a homotrimer that is able to interact with and stimulate the activity of a PCNA-interacting peptide-motif protein from E. histolytica, EhDNAligI. The data indicate a conservation of the biochemical mechanisms of PCNA-mediated interactions between metazoa, yeast and parasitic protozoa.
增殖细胞核抗原（PCNA）是一种环形蛋白质，参与细胞周期调控、DNA复制和DNA修复。寄生原生动物是早期分化的真核生物，可引发被忽视的疾病。在这项研究中，一种来自寄生原生动物的PCNA被鉴定、克隆并进行了生化特性分析，同时确定了其晶体结构。结构和生化研究表明，溶组织内阿米巴的PCNA组装成同源三聚体，能够与溶组织内阿米巴的一种PCNA相互作用肽基序蛋白EhDNAligI相互作用并刺激其活性。数据表明后生动物、酵母和寄生原生动物之间PCNA介导的相互作用的生化机制具有保守性。
