Scarpini E, Lisak R P, Beretta S, Velicogna M, Doronzo R, Moggio M, Jann S, Scarlato G
Institute of Neurology, Dino Ferrari Centre, University of Milan, Italy.
Brain. 1990 Jun;113 ( Pt 3):659-75. doi: 10.1093/brain/113.3.659.
We have analysed class II molecules (HLA-DR) expression in human fetal nerves at different stages of gestation, in human control nerves and in nerves of patients with peripheral neuropathies of different aetiology. Immunochemical demonstration of HLA-DR antigens was also performed on isolated human Schwann cells at different times in culture, from the same nerves. In normal nerves, HLA-DR positive material is localized only on endothelial and perivascular cells and in rare perineurial and endoneurial cells. In fetal nerves, HLA-DR positive material is already present by the sixteenth week of gestation. Quantitative analysis of HLA-DR molecules density in pathological nerves showed a wide heterogeneity in different neuropathies with no correlation between the type of pathology and the intensity and pattern of staining. Cultured human Schwann cells obtained from fetal, normal adult and pathological (vasculitic neuropathy) nerves were HLA-DR positive and the percentage of positive cells increased with time in culture. DR antigen appearance on human Schwann cells may reflect not only an immunologically-mediated phenomenon but also a dependence on axon-Schwann cell interaction.
我们分析了妊娠不同阶段的人类胎儿神经、人类对照神经以及不同病因的周围神经病患者神经中II类分子（HLA - DR）的表达情况。还对来自相同神经、处于不同培养时间的分离人雪旺细胞进行了HLA - DR抗原的免疫化学鉴定。在正常神经中，HLA - DR阳性物质仅定位于内皮细胞和血管周围细胞以及罕见的神经束膜细胞和神经内膜细胞。在胎儿神经中，妊娠第16周时已出现HLA - DR阳性物质。对病理神经中HLA - DR分子密度的定量分析显示，不同神经病存在广泛的异质性，病理类型与染色强度和模式之间无相关性。从胎儿、正常成人及病理（血管炎性神经病）神经中获取的培养人雪旺细胞呈HLA - DR阳性，且阳性细胞百分比随培养时间增加。人雪旺细胞上DR抗原的出现可能不仅反映免疫介导现象，还反映对轴突 - 雪旺细胞相互作用的依赖性。
