Sommer C, Schröder J M
Institut für Neuropathologie, Klinikum der Rheinisch-Westfälischen Technischen Hochschule, Aachen, Germany.
Acta Neuropathol. 1995;89(1):63-71. doi: 10.1007/BF00294261.
The expression of HLA-DR and the macrophage marker CD 68 was studied in 44 sural nerve biopsies from patients with inflammatory and non-inflammatory neuropathies and controls using immunohistochemistry on non-osmicated semithin sections, a technique that has not been used before in such a biopsy study. Most HLA-DR-immunoreactive (ir) cells were fibroblasts, macrophages or perineural cells, some were perivascular and endothelial cells, and only few were Schwann cells. Counts of immunoreactive cells revealed (a) increased HLA-DR expression in severe as compared to less severe neuropathies and to controls, (b) no correlation between the numbers of HLA-DR-ir cells and CD 68-ir macrophages, and (c) no close correlation between diagnostic groups and the number of HLA-DR-ir cells, but higher numbers in inflammatory neuropathies. We conclude that endoneurial fibroblasts and macrophages as antigen-presenting cells may be mediators in various peripheral nerve diseases, not only in inflammatory disorders.
采用免疫组织化学方法，在未经锇酸盐处理的半薄切片上，对44例炎性和非炎性神经病患者及对照者的腓肠神经活检组织进行研究，观察HLA - DR和巨噬细胞标志物CD 68的表达情况，这一技术此前尚未用于此类活检研究。大多数HLA - DR免疫反应性（ir）细胞为成纤维细胞、巨噬细胞或神经周细胞，部分为血管周围细胞和内皮细胞，仅有少数为施万细胞。免疫反应性细胞计数显示：（a）与病情较轻的神经病及对照相比，重症神经病中HLA - DR表达增加；（b）HLA - DR - ir细胞数量与CD 68 - ir巨噬细胞数量之间无相关性；（c）诊断组与HLA - DR - ir细胞数量之间无密切相关性，但炎性神经病中的细胞数量较多。我们得出结论，作为抗原呈递细胞的神经内膜成纤维细胞和巨噬细胞可能是各种周围神经疾病的介质，不仅在炎性疾病中如此。
