Universidade Federal do Tocantins, Centro Universitário de Porto Nacional, Porto Nacional, TO Brazil.
Genet Mol Biol. 2009 Jul;32(3):538-45. doi: 10.1590/S1415-47572009005000059. Epub 2009 Sep 1.
The aim of this study was to evaluate methods for developing a Brazilian maize core collection. For an initial survey of the active collection, passport information, as well as characterization and evaluation of accessions, were taken into consideration, these then being divided according to geographic region and kernel-type. Multiple sampling methods were evaluated. The strategy of constant sampling generated extensive alterations in extract accession frequency. The multivariate strategy with dispersion graphs and principal components associated with the Tocher method was considered efficient for identifying the most divergent genotypes. The multivariate strategy generated greater alterations in the variance of traits. The average number of traits revealed few modifications with the various sampling strategies used. Therefore, the active collection could be considered as possessing a satisfactory amount of information for most of its accessions. Moreover, the multivariate strategy generated modifications in the variance of the traits, independent of sampling intensity.
本研究旨在评估开发巴西玉米核心种质库的方法。对于初步的主动收集调查，考虑了护照信息，以及对材料的特征和评价，然后根据地理位置和籽粒类型进行分类。评估了多种抽样方法。固定抽样策略导致提取材料频率发生广泛变化。结合散点图和主成分的多元策略与 Tocher 方法相结合，被认为是识别最具差异基因型的有效方法。多元策略导致性状方差发生更大变化。使用各种抽样策略，平均性状数量变化不大。因此，对于大多数材料，主动收集可以被认为具有足够的信息量。此外，多元策略导致性状方差的变化，而与抽样强度无关。
