Nayak Spurthi N, Song Jian, Villa Andrea, Pathak Bhuvan, Ayala-Silva Tomas, Yang Xiping, Todd James, Glynn Neil C, Kuhn David N, Glaz Barry, Gilbert Robert A, Comstock Jack C, Wang Jianping
Agronomy Department, University of Florida, Gainesville, Florida, United States of America.
Agronomy Department, University of Florida, Gainesville, Florida, United States of America; College of Life Sciences, Dezhou University, Dezhou, Shandong, China.
PLoS One. 2014 Oct 21;9(10):e110856. doi: 10.1371/journal.pone.0110856. eCollection 2014.
Sugarcane (Saccharum spp.) and other members of Saccharum spp. are attractive biofuel feedstocks. One of the two World Collections of Sugarcane and Related Grasses (WCSRG) is in Miami, FL. This WCSRG has 1002 accessions, presumably with valuable alleles for biomass, other important agronomic traits, and stress resistance. However, the WCSRG has not been fully exploited by breeders due to its lack of characterization and unmanageable population. In order to optimize the use of this genetic resource, we aim to 1) genotypically evaluate all the 1002 accessions to understand its genetic diversity and population structure and 2) form a core collection, which captures most of the genetic diversity in the WCSRG. We screened 36 microsatellite markers on 1002 genotypes and recorded 209 alleles. Genetic diversity of the WCSRG ranged from 0 to 0.5 with an average of 0.304. The population structure analysis and principal coordinate analysis revealed three clusters with all S. spontaneum in one cluster, S. officinarum and S. hybrids in the second cluster and mostly non-Saccharum spp. in the third cluster. A core collection of 300 accessions was identified which captured the maximum genetic diversity of the entire WCSRG which can be further exploited for sugarcane and energy cane breeding. Sugarcane and energy cane breeders can effectively utilize this core collection for cultivar improvement. Further, the core collection can provide resources for forming an association panel to evaluate the traits of agronomic and commercial importance.
甘蔗（甘蔗属）及甘蔗属的其他成员是有吸引力的生物燃料原料。两个世界甘蔗及相关禾本科植物种质资源库（WCSRG）之一位于佛罗里达州迈阿密。这个WCSRG有1002份种质，可能具有与生物量、其他重要农艺性状和抗逆性相关的宝贵等位基因。然而，由于缺乏特征描述和难以管理的群体规模，育种者尚未充分利用该WCSRG。为了优化这种遗传资源的利用，我们旨在：1）对所有1002份种质进行基因型评估，以了解其遗传多样性和群体结构；2）形成一个核心种质库，该种质库能捕获WCSRG中的大部分遗传多样性。我们在1002个基因型上筛选了36个微卫星标记，并记录了209个等位基因。WCSRG的遗传多样性范围为0至0.5，平均为0.304。群体结构分析和主坐标分析揭示了三个聚类，所有的野生种甘蔗聚在一个聚类中，热带种甘蔗和杂交种甘蔗聚在第二个聚类中，而第三个聚类主要是非甘蔗属物种。确定了一个由300份种质组成的核心种质库，该种质库捕获了整个WCSRG的最大遗传多样性，可进一步用于甘蔗和能源甘蔗育种。甘蔗和能源甘蔗育种者可以有效地利用这个核心种质库来改良品种。此外，该核心种质库可为组建关联群体提供资源，以评估具有农艺和商业重要性的性状。
