巴西伊瓦伊河上巴拉那河上游地区两个里氏鲶属（Siluriformes, Loricariidae）种群的同工酶比较。

Allozyme comparison of two populations of Rineloricaria (Siluriformes, Loricariidae) from the Ivaí River, upper Paraná River basin, Brazil.

机构信息

Curso de Pós-graduação em Genética e Melhoramento, Universidade Estadual de Maringá, Maringá, PR Brazil.

出版信息

Genet Mol Biol. 2009 Apr;32(2):431-5. doi: 10.1590/S1415-47572009005000022. Epub 2009 Jan 30.

DOI:10.1590/S1415-47572009005000022

PMID:21637702

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3036927/

Abstract

Two allopatric morphotypes of the genus Rinelocaria were compared through the allozyme electrophoresis technique: one morphotype, R. pentamaculata, from the Keller River in the middle stretch of the Ivaí River basin and the other, R. aff. pentamaculata, from the São João River in the upper portion of the Ivaí River basin. The morphotype from the São João River was collected upstream from the São João waterfall, which is about 80 m deep. Twelve enzymatic systems (AAT, ADH, EST, GCDH, G3PDH, GPI, IDH, LDH, MDH, ME, PGM and SOD) were analyzed, which allowed to score 22 loci. Only lociAat-2, Est-3 and Mdh-C showed polymorphism. The two samples differed in allele frequencies at the three polymorphic loci. The average expected heterozygosity for all loci was 0.0806 ± 0.0447 in the Keller River sample. For the São João River morphotype, this value was 0.0489 ± 0.0350. Nei' s genetic identity and distance between the two populations were respectively 0.9789 and 0.0213. Wright's F(IS) , F(IT) and F(ST) over all loci were estimated as 0.3121, 0.4021 and 0.1309, respectively. We consider that the two morphotypes represent species in statunascendi.

摘要

两种不同生境的 Rinelocaria 属形态型通过同工酶电泳技术进行了比较：一种形态型是来自伊瓦伊河中游凯勒河的 R. pentamaculata，另一种是来自伊瓦伊河上游圣若昂河的 R. aff. pentamaculata。圣若昂河的形态型是在圣若昂瀑布的上游采集的，瀑布大约有 80 米深。分析了 12 个酶系统（AAT、ADH、EST、GCDH、G3PDH、GPI、IDH、LDH、MDH、ME、PGM 和 SOD），可以对 22 个基因座进行评分。只有 Aat-2、Est-3 和 Mdh-C 这三个基因座显示出多态性。在这三个多态性基因座上，两个样本的等位基因频率存在差异。在凯勒河样本中，所有基因座的平均预期杂合度为 0.0806 ± 0.0447。对于圣若昂河形态型，这一数值为 0.0489 ± 0.0350。两个种群之间的 Nei' 遗传同一性和遗传距离分别为 0.9789 和 0.0213。Wright 的 F(IS)、F(IT)和 F(ST)在所有基因座上的估计值分别为 0.3121、0.4021 和 0.1309。我们认为这两种形态型代表了处于上升状态的物种。