多维G-四链体解链曲线分析
Analysis of multidimensional G-quadruplex melting curves.
作者信息
Gray Robert D, Chaires Jonathan B
机构信息
James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky, USA.
出版信息
Curr Protoc Nucleic Acid Chem. 2011 Jun;Chapter 17:Unit17.4. doi: 10.1002/0471142700.nc1704s45.
Abstract
Multidimensional "3D" melting curves for G-quadruplexes are obtained by recording whole spectra (absorbance, CD, fluorescence) as a function of temperature, rather than the common approach of recording the spectral response to temperature at a single wavelength. 3D melting curves are richer in information, and can be used to enumerate the number of significant species and intermediate states required to properly analyze the thermal denaturation reaction to obtain thermodynamic information. This unit describes the application of the method of singular value decomposition to the analysis of 3D melting data obtained for G-quadruplex structures, and how the results of such an analysis can be used to provide a more complete characterization of the mechanism of quadruplex unfolding.
摘要
通过记录整个光谱（吸光度、圆二色性、荧光）随温度的变化来获得G-四链体的多维“3D”熔解曲线，而不是采用在单一波长下记录光谱对温度响应的常规方法。3D熔解曲线包含更丰富的信息，可用于确定正确分析热变性反应以获得热力学信息所需的重要物种和中间状态的数量。本单元描述了奇异值分解方法在分析G-四链体结构的3D熔解数据中的应用，以及这种分析结果如何用于更全面地表征四链体解折叠机制。
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