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Drugs. 2020 Jul;80(10):941-946. doi: 10.1007/s40265-020-01321-z.
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Temperature-controlled electrospray ionization mass spectrometry as a tool to study collagen homo- and heterotrimers.温控电喷雾电离质谱法作为研究胶原蛋白同三聚体和异三聚体的工具。
Chem Sci. 2019 Sep 3;10(42):9829-9835. doi: 10.1039/c9sc03248g. eCollection 2019 Nov 14.
4
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Chem Commun (Camb). 2020 Jan 28;56(9):1317-1324. doi: 10.1039/c9cc09107f. Epub 2020 Jan 6.
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Molecules. 2019 Oct 21;24(20):3781. doi: 10.3390/molecules24203781.
6
Dynamic split G-quadruplex programmed reversible nanodevice.动态分裂 G-四链体编程可逆纳器件。
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7
Submicrometer Nanospray Emitters Provide New Insights into the Mechanism of Cation Adduction to Anionic Oligonucleotides.亚微米纳喷雾发射器为阳离子加合到阴离子寡核苷酸的机制提供了新的见解。
Anal Chem. 2018 Nov 20;90(22):13541-13548. doi: 10.1021/acs.analchem.8b03632. Epub 2018 Nov 5.
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J Am Chem Soc. 2018 Oct 3;140(39):12553-12565. doi: 10.1021/jacs.8b07302. Epub 2018 Sep 18.
9
G-Quadruplex Secondary Structure Obtained from Circular Dichroism Spectroscopy.从圆二色性光谱获得的 G-四链体二级结构。
Angew Chem Int Ed Engl. 2018 Jun 11;57(24):7171-7175. doi: 10.1002/anie.201709184. Epub 2018 Apr 25.
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从温度控制的电喷雾电离质谱中获得对近端 DNA 结构域相互作用的新认识。

Novel Insight into Proximal DNA Domain Interactions from Temperature-Controlled Electrospray Ionization Mass Spectrometry.

机构信息

Department of Chemistry and Applied Biosciences, ETH Zurich, Vladimir-Prelog-Weg 3, 8093, Zurich, Switzerland.

出版信息

Angew Chem Int Ed Engl. 2021 Jul 5;60(28):15390-15398. doi: 10.1002/anie.202016757. Epub 2021 May 26.

DOI:10.1002/anie.202016757
PMID:33822450
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8251475/
Abstract

Quadruplexes are non-canonical nucleic acid structures essential for many cellular processes. Hybrid quadruplex-duplex oligonucleotide assemblies comprised of multiple domains are challenging to study with conventional biophysical methods due to their structural complexity. Here, we introduce a novel method based on native mass spectrometry (MS) coupled with a custom-built temperature-controlled nanoelectrospray ionization (TCnESI) source designed to investigate interactions between proximal DNA domains. Thermal denaturation experiments were aimed to study unfolding of multi-stranded oligonucleotide constructs derived from biologically relevant structures and to identify unfolding intermediates. Using the TCnESI MS, we observed changes in T and thermodynamic characteristics of proximal DNA domains depending on the number of domains, their position, and order in a single experiment.

摘要

四链体是非经典核酸结构，对于许多细胞过程至关重要。由于其结构复杂性，使用传统的生物物理方法很难研究由多个结构域组成的杂交四链体-双链体寡核苷酸组装体。在这里，我们介绍了一种基于天然质谱（MS）的新方法，该方法与定制的温度控制纳喷雾电离（TCnESI）源相结合，旨在研究近端 DNA 结构域之间的相互作用。热变性实验旨在研究源自生物相关结构的多链寡核苷酸结构的展开，并鉴定展开中间体。使用 TCnESI MS，我们观察到取决于域的数量、它们的位置和在单个实验中的顺序的近端 DNA 域的 T 和热力学特性的变化。