Paulino Tony P, Magalhães Prislaine P, Thedei Geraldo, Tedesco Antônio C, Ciancaglini Pietro
Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão, Preto, FFCLRP-USP, 14040-901 Ribeirão Preto, SP, Brasil.
Biochem Mol Biol Educ. 2005 Jan;33(1):46-9. doi: 10.1002/bmb.2005.494033010424.
The main focus of this laboratory exercise was to investigate the photodynamic therapy (PDT) acting over Streptococcus mutans. A handheld photopolymerizer and a classical photosensitizer (Rose Bengal) were used to induce photodynamic response. In this way, a suspension of S. mutans was treated with different concentrations of Rose Bengal (0-10 μmol/liter), irradiated with a light (400-600 nm) for 20 s, and then cell viability was evaluated. It was observed that the light (per se) is not toxic, and in the dark, Rose Bengal is toxic only to the cells tested at concentrations above 5.0 μmol/liter. Under light exposure, concentrations of Rose Bengal above 0.5 μmol/liter killed all S. mutans. Therefore, for the purpose of our work, the photoactivation of Rose Bengal using the handheld photopolymerizer was efficient in bacteria inactivation.
本实验的主要重点是研究光动力疗法（PDT）对变形链球菌的作用。使用手持式光固化器和经典光敏剂（孟加拉玫瑰红）来诱导光动力反应。通过这种方式，用不同浓度的孟加拉玫瑰红（0 - 10 μmol/升）处理变形链球菌悬液，用400 - 600 nm的光照射20秒，然后评估细胞活力。观察到光（本身）无毒，在黑暗中，孟加拉玫瑰红仅对浓度高于5.0 μmol/升的受试细胞有毒。在光照下，浓度高于0.5 μmol/升的孟加拉玫瑰红可杀死所有变形链球菌。因此，就我们的工作而言，使用手持式光固化器对孟加拉玫瑰红进行光活化在细菌灭活方面是有效的。
