使用激光或发光二极管的光动力疗法对致龋菌的比较评估：一项体外研究。
Comparative evaluation of photodynamic therapy using LASER or light emitting diode on cariogenic bacteria: An in vitro study.
作者信息
Ricatto Liliana Guimarães Oliveira, Conrado Luis Augusto Lopato, Turssi Cecilia Pedroso, França Fabiana Mantovani Gomes, Basting Roberta Tarkany, Amaral Flávia Lucisano Botelho
机构信息
Department of Restorative Dentistry, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo, Brazil.
Department of Biomedical Engineering, Unicastelo, São José dos Campos, São Paulo, Brazil.
出版信息
Eur J Dent. 2014 Oct;8(4):509-514. doi: 10.4103/1305-7456.143634.
OBJECTIVE
The aim of this study was to evaluate in vitro the effect of photodynamic therapy (PDT) using LASER or light emitting diode (LED) on cariogenic bacteria (Streptococcus mutans [SM] and Lactobacillus casei [LC]) in bovine dentin.
MATERIALS AND METHODS
Twenty five fragments of dentin were contaminated with SM and LC strands and divided into five experimental groups according to the therapy they received (n = 5): C - control (no treatment), SCLED - no dye/LED application (94 J/cm(2)), SCLASER - no dye/LASER application (94 J/cm(2)), CCLED - dye/LED application (94 J/cm(2)) and CCLASER - dye/LASER application (94 J/cm(2)). The dye used was methylene blue at 10 mM. Dentin scrapes were harvested from each fragment and prepared for counts of colony forming units (CFU)/mL. The data were analyzed using Kruskal-Wallis, followed by Student-Newman-Keuls (α =0.05).
RESULTS
Regarding SM, groups CCLASER and CCLED showed a significant reduction in CFU/mL, which was statistically superior to the SCLASER, SCLED and C groups. Regarding LC, the groups CCLASER and CCLED caused a significant reduction in CFU/mL when compared with SCLASER, which showed intermediate values. SCLED and C had a lesser effect on reducing CFU/mL, where the former showed values similar to those of SCLASER.
CONCLUSIONS
In conclusion, PDT combined with LASER or LED and methylene blue had a significant antimicrobial effect on cariogenic bacteria in the dentin.
目的
本研究旨在体外评估使用激光或发光二极管（LED）的光动力疗法（PDT）对牛牙本质中致龋菌（变形链球菌[SM]和干酪乳杆菌[LC]）的影响。
材料与方法
25个牙本质碎片被SM和LC菌链污染，并根据接受的治疗方法分为五个实验组（n = 5）：C组 - 对照组（未治疗），SCLED组 - 不应用染料/LED（94 J/cm²），SCLASER组 - 不应用染料/激光（94 J/cm²），CCLED组 - 应用染料/LED（94 J/cm²）和CCLASER组 - 应用染料/激光（94 J/cm²）。使用的染料是10 mM的亚甲蓝。从每个碎片上收集牙本质刮屑并制备用于计算菌落形成单位（CFU）/mL。数据使用Kruskal-Wallis检验进行分析，随后进行Student-Newman-Keuls检验（α = 0.05）。
结果
关于SM，CCLASER组和CCLED组的CFU/mL显著降低，在统计学上优于SCLASER组、SCLED组和C组。关于LC，与显示中间值的SCLASER组相比，CCLASER组和CCLED组导致CFU/mL显著降低。SCLED组和C组对降低CFU/mL的效果较小，前者显示的值与SCLASER组相似。
结论
总之，PDT联合激光或LED以及亚甲蓝对牙本质中的致龋菌具有显著的抗菌作用。
Zotero 插件