Fujita Y, Fujita T, Miwa Y
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Japan.
FEBS Lett. 1990 Jul 2;267(1):71-4. doi: 10.1016/0014-5793(90)80290-y.
Transcription of the Bacillus subtilis gnt operon results in a polycistronic mRNA that encodes from the 5' end the Gnt repressor, gluconate kinase and permease. The RNA is drastically induced through inactivation of the Gnt repressor by gluconate. The results of deletion analysis of the gnt promoter region upstream of the repressor gene indicated that no other promoter except the gnt promoter was present for expression of the gluconate kinase gene. In contrast to the synthesis of gluconate kinase and permease, which was markedly induced by gluconate, the results of a radioimmunoassay for the Gnt repressor indicated that synthesis of the Gnt repressor from the induced mRNA was posttranscriptionally repressed.
枯草芽孢杆菌gnt操纵子的转录产生一种多顺反子mRNA，该mRNA从5'端开始编码Gnt阻遏蛋白、葡萄糖酸激酶和通透酶。葡萄糖酸通过使Gnt阻遏蛋白失活而强烈诱导该RNA的产生。对阻遏蛋白基因上游gnt启动子区域的缺失分析结果表明，除gnt启动子外，不存在其他用于葡萄糖酸激酶基因表达的启动子。与葡萄糖酸显著诱导的葡萄糖酸激酶和通透酶的合成相反，Gnt阻遏蛋白的放射免疫分析结果表明，诱导mRNA产生的Gnt阻遏蛋白的合成在转录后受到抑制。
