Wang J, Rivas G, Ozsoz M, Grant D H, Cai X, Parrado C
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003.
Anal Chem. 1997 Apr 1;69(7):1457-60. doi: 10.1021/ac961000d.
An electrochemical biosensor protocol for the detection of radiation-induced DNA damage is described. The procedure employs a dsDNA-coated screen-printed electrode and relies on changes in the guanine-DNA oxidation signal upon exposure to ultraviolet radiation. The decreased signal is ascribed primarily to conformational changes in the DNA and to the photoconversion of the guanine-DNA moiety to a nonelectroactive monomeric base product. Factors influencing the response of these microfabricated DNA sensors, such as irradiation time, wavelength, and distance, are explored, and future prospects are discussed. Similar results are given for the use of bare strip electrodes in connection with irradiated DNA solutions.
描述了一种用于检测辐射诱导的DNA损伤的电化学生物传感器方案。该程序采用涂有双链DNA的丝网印刷电极，并依赖于暴露于紫外线辐射时鸟嘌呤-DNA氧化信号的变化。信号降低主要归因于DNA的构象变化以及鸟嘌呤-DNA部分向非电活性单体碱基产物的光转化。探讨了影响这些微制造DNA传感器响应的因素，如照射时间、波长和距离，并讨论了未来前景。对于使用裸条形电极与辐照DNA溶液结合的情况，也给出了类似的结果。
