Tencaliec Anca Monica, Laschi Serena, Magearu Vasile, Mascini Marco
University of Bucharest, Faculty of Chemistry, Department of Analytical Chemistry, Blvd. Regina Elisabeta 4-12, Bucharest, Romania.
Talanta. 2006 Apr 15;69(2):365-9. doi: 10.1016/j.talanta.2005.09.042. Epub 2005 Nov 9.
In the present study, a comparison between a disposable electrochemical DNA biosensor and a Vibrio fischeri-based luminescent sensor for the detection of toxicants in water samples was made. In order to realize this study, a disposable electrochemical DNA biosensor has been reported. The DNA biosensor is assembled by immobilizing double stranded Calf Thymus DNA onto the surface of a disposable carbon screen-printed electrode. The oxidation signal of the guanine base, obtained by a square wave voltammetric scan, is used as analytical signal to detect the DNA damage; the presence of low molecular weight compounds with affinity for nucleic acids is measured by their effect on the guanine oxidation peak. Wastewater samples provided during First European Interlaboratory Exercise on water toxicity in the course of the project SWIFT-WFD were analyzed, and biosensor results were compared with a currently used toxicity test ToxAlert 100 based on the bioluminescence inhibition of Vibrio fischeri. This test have been used because is rapid, easy handling and cost effectively responses for the toxicity assessment in real water samples. The results showed a promising correlation between two tests used for the detection of toxic compounds in water samples.
在本研究中，对用于检测水样中有毒物质的一次性电化学DNA生物传感器和基于费氏弧菌的发光传感器进行了比较。为实现本研究，已报道了一种一次性电化学DNA生物传感器。该DNA生物传感器是通过将双链小牛胸腺DNA固定在一次性碳丝网印刷电极表面组装而成。通过方波伏安扫描获得的鸟嘌呤碱基的氧化信号用作检测DNA损伤的分析信号；通过它们对鸟嘌呤氧化峰的影响来测量对核酸具有亲和力的低分子量化合物的存在。对项目SWIFT-WFD过程中第一次欧洲水毒性实验室间比对活动提供的废水样本进行了分析，并将生物传感器的结果与目前使用的基于费氏弧菌生物发光抑制的毒性测试ToxAlert 100进行了比较。之所以使用该测试，是因为它快速、易于操作且对实际水样中的毒性评估具有成本效益高的响应。结果表明，用于检测水样中有毒化合物的两种测试之间存在有前景的相关性。
