Laboratory of Chromosome and Cell Biology, Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
FEBS Lett. 2011 Sep 16;585(18):2876-82. doi: 10.1016/j.febslet.2011.05.046. Epub 2011 Jun 1.
The Ku heterodimer, consisting of the proteins Ku70 and Ku80, is the central component of the non-homologous end joining (NHEJ) pathway of double strand break (DSB) repair. Ku is able to recognize and bind a DSB by virtue of its ring-like structure. Both pre-repair and topologically trapped post-repair Ku heterodimers are thought to be inhibitory to multiple cellular processes. Thus, a regulated mechanism for the removal of Ku from chromatin was predicted to exist. Recent evidence shows that Ku80 is removed from DNA through a ubiquitin-mediated process. Similar processes have been shown to be involved in the regulated dissociation of a host of other proteins from chromatin, and this appears to be a general and conserved mechanism for the regulation of chromatin-associated factors. A potential mechanism for this pathway is discussed.
Ku 异源二聚体由 Ku70 和 Ku80 蛋白组成，是双链断裂 (DSB) 修复中非同源末端连接 (NHEJ) 途径的核心组成部分。Ku 凭借其环状结构能够识别和结合 DSB。预修复和拓扑学上困住的修复后 Ku 异源二聚体都被认为对多种细胞过程具有抑制作用。因此，预测存在一种用于将 Ku 从染色质中去除的调节机制。最近的证据表明，Ku80 通过泛素介导的过程从 DNA 中去除。类似的过程已被证明涉及许多其他蛋白质从染色质上的调节解离，这似乎是调节染色质相关因子的一种普遍和保守的机制。讨论了该途径的一种潜在机制。
