Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, Taiwan.
J Virol Methods. 2011 Aug;175(2):175-81. doi: 10.1016/j.jviromet.2011.05.006. Epub 2011 May 27.
Avian influenza virus (AIV) is an enveloped virus with segmented RNA that belongs to the Orthomyxoviridae. Recently, avian influenza virus isolates have not only posed a significant threat to the poultry industry but also serious public health concerns. The full-length viral hemagglutinin (HA), neuraminidase (NA) or both genes were inserted into the yeast Kluyveromyces lactis genome to allow for secreted expression. Both hemagglutinin and neuraminidase activities were demonstrated for the expressed proteins. Based on PNGase F digestion and immunoassays, N-glycosidically linked high mannose or hybrid-type carbohydrate chains on the HA protein are predominant. It is noteworthy that when co-expression of the HA and NA proteins was carried out, the NA protein was able to react with the HA protein, resulting in deglycosylation in a manner similar to PNGase F digestion. Such post-translational modifications in the HA and NA proteins of AIV are described for the first time.
禽流感病毒（AIV）是一种带有分段 RNA 的包膜病毒，属于正粘病毒科。最近，禽流感病毒分离株不仅对家禽业构成了重大威胁，也对公共卫生造成了严重关注。全长病毒血凝素（HA）、神经氨酸酶（NA）或两者的基因被插入酵母克鲁维酵母基因组中，以允许分泌表达。表达的蛋白均显示出血凝素和神经氨酸酶活性。基于 PNGase F 消化和免疫测定，HA 蛋白上的 N-糖基化高甘露糖或杂合型碳水化合物链占主导地位。值得注意的是，当 HA 和 NA 蛋白共同表达时，NA 蛋白能够与 HA 蛋白反应，导致类似 PNGase F 消化的去糖基化。AIV 的 HA 和 NA 蛋白的这种翻译后修饰是首次描述的。
