Environmental Microbial Genomics Group, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 avenue Guy de Collongue 69134 Ecully, France.
J Microbiol Methods. 2011 Aug;86(2):255-7. doi: 10.1016/j.mimet.2011.05.014. Epub 2011 May 27.
Gene transfer frequency can be determined experimentally on plates, but the methods currently in use do not discriminate between independent transfers and clonal multiplication of initial transformants. In order to overcome this bias, we engineered an Acinetobacter baylyi population in which cells differed by a specific molecular signature and used it as recipient in transformation experiments. Our results suggest that a corrective factor of 0.52 should be applied in order to accurately report natural transformation when using the plate counting method.
基因转移频率可以在平板上进行实验测定，但目前使用的方法无法区分独立转移和初始转化体的克隆增殖。为了克服这种偏差，我们构建了一个具有特定分子特征的鲍曼不动杆菌种群，并将其用作转化实验的受体。我们的结果表明，当使用平板计数法时，应该应用 0.52 的校正因子来准确报告自然转化。
