Institute of Biological Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia.
J Ethnopharmacol. 2011 Sep 1;137(1):257-62. doi: 10.1016/j.jep.2011.05.013. Epub 2011 May 24.
Evaluations of the anti-snake venom efficacy of Mimosa pudica tannin isolate (MPT) obtained from root of the plant.
MPT was investigated in vitro and in vivo for its efficacy against the venom of Naja kaouthia snake.
In vitro: (1) mice injected i.p. with MPT pre-incubated with Naja kaouthia venom at concentrations as low as 0.625 mg/ml showed 100% survival after a 24-h observation period. (2) In the proteomics study, mice injected with MPT pre-incubated with the Naja kaouthia venom showed down-regulation of five serum proteins. (3) In the protein-dye-binding study, the percentage of Bradford dye-protein binding showed a reduction relative to the decrease in MPT concentration used to incubate with the venom. In vivo: the results from the animal studies showed that MPT had no in vivo protection against the Naja kaouthia venom (0.875 mg/kg) in four different rescue modes and in an oral pre-treatment experiment.
The study indicated the promising ability of MPT to neutralize the Naja kaouthia venom in in vitro experiments but fell short in its in vivo potential. As such, the use of Mimosa pudica (Mimosaceae) as therapeutics for snake bites is questionable as all the possible in vivo rescue studies and pre-treatment of the active constituents showed no protection against the affected mice.
评估从植物根部获得的含羞草单宁分离物（MPT）对眼镜蛇毒液的抗蛇毒功效。
研究了 MPT 对眼镜蛇毒液的体内和体外疗效。
在体外：（1）用 MPT 预先孵育的 Naja kaouthia 毒液，浓度低至 0.625mg/ml 的小鼠腹腔注射后，在 24 小时观察期后 100%存活。（2）在蛋白质组学研究中，用 MPT 预先孵育眼镜蛇毒液的小鼠显示 5 种血清蛋白下调。（3）在蛋白染料结合研究中，Bradford 染料-蛋白结合的百分比显示与用于与毒液孵育的 MPT 浓度降低相对应的减少。在体内：动物研究结果表明，MPT 在四种不同的抢救模式和口服预处理实验中，对 Naja kaouthia 毒液（0.875mg/kg）没有体内保护作用。
该研究表明 MPT 在体外实验中具有中和眼镜蛇毒液的潜力，但在体内潜力方面存在不足。因此，含羞草（含羞草科）作为蛇咬伤的治疗方法是值得怀疑的，因为所有可能的体内抢救研究和活性成分的预处理都没有对受影响的小鼠提供保护。
