School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA.
Fungal Biol. 2011 Jun;115(6):485-92. doi: 10.1016/j.funbio.2011.02.012. Epub 2011 Feb 22.
To date, among the zygomycete fungi that have been examined, a Spitzenkörper has not been reported. In this paper, the cytoplasmic order of hyphal tip cells of Basidiobolus sp., a zygomycete genus of uncertain phylogeny, has been examined using light microscopy and transmission electron microscopy methods. With phase-contrast light optics, a phase-dark body was observed at the tips of growing hyphae of Basidiobolus sp. The hyphal apex also showed high affinity for FM4-64 labelling resulting in an intense fluorescence signal. The phase-dark inclusion exhibited independent motility within the hyphal apex and its presence and position were correlated to the rate and direction of hyphal growth. The hyphal apex of Basidiobolus sp. did not contain γ-tubulin. Ultrastructural observations revealed a dense cluster of vesicles at the hyphal apex. These results suggest that the growing hypha of Basidiobolus sp. contains a Spitzenkörper, a character generally attributed to members of the ascomycete and basidiomycete fungi and not to zygomycete fungi.
迄今为止，在已研究的蕈菌真菌中，尚未报道过尖状体。本文采用光学显微镜和透射电子显微镜方法，对分类学上不确定的蕈菌属 Basidiobolus 的菌丝顶端细胞的细胞质秩序进行了研究。在相差光镜下，在 Basidiobolus sp.的生长菌丝的顶端观察到一个相暗体。菌丝顶端对 FM4-64 标记也表现出高亲和力，导致强烈的荧光信号。相暗包含物在菌丝顶端内具有独立的运动性，其存在和位置与菌丝生长的速度和方向相关。Basidiobolus sp.的菌丝顶端不含γ-微管蛋白。超微结构观察显示，在菌丝顶端有一个密集的囊泡簇。这些结果表明，Basidiobolus sp.的生长菌丝含有尖状体，这一特征通常归因于子囊菌和担子菌真菌，而不是蕈菌真菌。
