Fisher Karen E, Roberson Robert W
School of Life Sciences, Arizona State University, Tempe, Arizona 85287.
School of Life Sciences, Arizona State University, Tempe, Arizona 85287
Mycologia. 2016 May-Jun;108(3):533-42. doi: 10.3852/15-226. Epub 2016 Feb 23.
We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitution methods to preserve fungal hyphae for transmission electron microscopy, which yielded improved preservation of ultrastructural details. Our research has confirmed studies that described the accumulation of secretory vesicles as a crescent at the hyphal apex (i.e. the apical vesicle crescent [AVC]) and provided a more detailed understanding of the vesicle populations. In addition, we have been able to observe the behavior of the AVC during hyphal growth in M. indicus and G. persicaria.
我们利用光学显微镜和透射电子显微镜，研究了四种接合菌纲真菌的菌丝尖端结构：轮枝状被孢霉（被孢霉目）、反卷科曼斯霉（梳霉目）、印度毛霉和桃吉尔霉（毛霉目）。我们采用冷冻固定和冷冻置换方法来保存用于透射电子显微镜观察的真菌菌丝，这使得超微结构细节得到了更好的保存。我们的研究证实了之前的研究，即分泌囊泡在菌丝顶端积累形成新月形（即顶端囊泡新月体[AVC]），并对囊泡群体有了更详细的了解。此外，我们还能够观察到印度毛霉和桃吉尔霉菌丝生长过程中AVC的行为。
