Hukkanen V, Ziegler T, Kinnunen L, Hirvonen H, Auvinen P, Hovi T
Department of Virology, University of Turku, Finland.
Mol Cell Probes. 1990 Apr;4(2):81-5. doi: 10.1016/0890-8508(90)90010-w.
Genomic variation of herpes simplex virus type 2 (HSV-2) strains was analysed by polyacrylamide gradient gel electrophoresis and subsequent hybridization to cloned HSV-2 sequences. Two probes were used, one from the L-segment and one from the S-segment of the HSV-2 genome. The probes did not contain a-repeat sequences. Hybridization to the specific sequences in individual DNA fragments obtained by use of the frequently cleaving restriction endonuclease Alu I revealed variations in the genome not detectable by analysing the fragment size only. The use of 35S-labelled deoxynucleotide in the radioactive labelling of the probe further improved the resolution of the method.
