Sakaoka H, Kawana T, Grillner L, Aomori T, Yamaguchi T, Saito H, Fujinaga K
J Gen Virol. 1987 Aug;68 ( Pt 8):2105-16. doi: 10.1099/0022-1317-68-8-2105.
One-hundred and twenty-three epidemiologically unrelated strains of herpes simplex virus type 2 (HSV-2) isolated in Japan and Sweden (68 Japanese and 55 Swedish isolates) were compared by analysis of their genomes using five restriction endonucleases: BamHI, KpnI, EcoRI, HindIII and Bg/II. Seven of the 93 restriction sites examined showed statistically significant variation between isolates from the two countries. However, HSV-2 isolates were less variable than the HSV-1 isolates previously analysed from the same countries. Using 12 restriction sites as markers, the HSV-2 isolates were classified into 41 cleavage patterns; 17 were specific for Japanese isolates and 15 were specific for Swedish isolates. Correlation coefficients between some sets of 12 markers were significant, but significant correlations between Japanese and Swedish isolates were distinct for each country. Both Japanese and Swedish isolates were assigned to three major patterns with no significant difference in incidence. In contrast, in two other major patterns, differences in incidence between the isolates were statistically significant. These results suggest that HSV-2 populations in geographically separated countries have distinct cleavage site distributions.
通过使用五种限制性内切酶（BamHI、KpnI、EcoRI、HindIII和Bg/II）对其基因组进行分析，比较了在日本和瑞典分离出的123株流行病学上无关的2型单纯疱疹病毒（HSV-2）（68株日本分离株和55株瑞典分离株）。在检测的93个限制性位点中，有7个在两国的分离株之间表现出统计学上的显著差异。然而，HSV-2分离株的变异性低于先前从同一国家分析的HSV-1分离株。以12个限制性位点作为标记，HSV-2分离株被分为41种切割模式；17种是日本分离株特有的，15种是瑞典分离株特有的。几组12个标记之间的相关系数是显著的，但日本和瑞典分离株之间的显著相关性在每个国家都是不同的。日本和瑞典的分离株都被归为三种主要模式，发病率没有显著差异。相比之下，在其他两种主要模式中，分离株之间的发病率差异具有统计学意义。这些结果表明，地理上分离的国家中的HSV-2群体具有不同的切割位点分布。
