Heywood L A, Burke J F
Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton, Great Britain.
Mutat Res. 1990 Jul;236(1):59-66. doi: 10.1016/0921-8777(90)90033-2.
Synthetic DNA linkers containing a single mismatched nucleotide (C:A) are repaired without bias at high efficiency when introduced into mammalian cells on a SV40 shuttle vector. From the pattern of repair in vectors containing multiple linkers, it appears that DNA synthesis following mismatch excision can replace a length of DNA as short as 40 nucleotides. Furthermore, results from the introduction of linker molecules containing combinations of single-strand nicks suggest that transient unsealed nicks do not drive the direction of mismatch repair in mammalian cells, as has previously been proposed.
当含有单个错配核苷酸（C:A）的合成DNA接头通过SV40穿梭载体导入哺乳动物细胞时，它们能被高效且无偏向性地修复。从含有多个接头的载体中的修复模式来看，错配切除后的DNA合成似乎能够替换短至40个核苷酸长度的DNA。此外，引入含有单链切口组合的接头分子的结果表明，短暂未封闭的切口并不像之前所提出的那样驱动哺乳动物细胞中错配修复的方向。
