Boldyrev A A
Biokhimiia. 1978 Nov;43(11):2100-5.
Temperature dependence of bovine brain NA,K-ATPase before and after the short-term treatment of enzyme preparations with phospholipases A, C and D is investigated. Arrhenius plots of the temperature dependence of the reaction rate catalysed by Na,K-ATPase are non-linear, they have an inflection at the region of about 20 degrees C. The treatment of the enzyme with phospholipase A makes the inflection more smooth, phospholipase D shifts the inflection by 4 degrees C to lower temperature and simultaneously activates Na,K-ATPase. Phospholipase C sharply changes the Arrhenius curve and makes it linear. The data obtained are discussed with respect to the role of phospholipids in the formation of membrane bilayer and in the regulation of Na,K-ATPase activity.
研究了用磷脂酶A、C和D对牛脑钠钾-ATP酶制剂进行短期处理前后该酶的温度依赖性。由钠钾-ATP酶催化的反应速率的温度依赖性的阿累尼乌斯曲线是非线性的，在约20℃区域有一个拐点。用磷脂酶A处理该酶使拐点更平滑，磷脂酶D使拐点向低温方向移动4℃并同时激活钠钾-ATP酶。磷脂酶C使阿累尼乌斯曲线急剧变化并使其变为线性。就磷脂在膜双层形成和钠钾-ATP酶活性调节中的作用对所获得的数据进行了讨论。
