Esyrev O V, Danilenko M P
Vopr Med Khim. 1980 Nov-Dec;26(6):778-81.
Microsomal preparations of Na, K-ATPase, isolated from dog and bovine brains, were completely insensitive to acetylcholine (10(-6)-10(-2) M) under standard experimental conditions. At the same time, heat pretreatment of membranes induced alterations of the enzymatic activity in presence of low concentrations (10(-6)-10(-5) M) of the neurotransmitter. Acetylcholine stimulated the Na, K-ATPase activity after storage of the preparations at 22 degrees within 30 or 60 min inhibited the activity at 37 degrees. Addition of the transmitter during the preincubation increased its effect. The maximal values of Na, K-ATPase activation and inhibition constituted 30% and 24%, respectively. D-tubocurarine (10(-6)-10(-5) M) and atropine (10(-4) M) did not eliminate and eserine (10(-4) M) did not alter the induced effect of acetylcholine. The effects of acetylcholine were changed to the opposite ones within 70-90 days of storage of the preparations. The phenomenon might be simulated by "ageing" of microsomes at 37 degrees within 3-6 hrs.
从狗和牛脑中分离出的钠钾 - ATP酶微粒体制剂，在标准实验条件下对乙酰胆碱（10⁻⁶ - 10⁻²M）完全不敏感。同时，膜的热预处理在低浓度（10⁻⁶ - 10⁻⁵M）神经递质存在下诱导酶活性改变。乙酰胆碱在制剂于22℃储存30或60分钟后刺激钠钾 - ATP酶活性，在37℃时抑制活性。预孵育期间添加递质会增强其作用。钠钾 - ATP酶激活和抑制的最大值分别为30%和24%。筒箭毒碱（10⁻⁶ - 10⁻⁵M）和阿托品（10⁻⁴M）不能消除，而毒扁豆碱（10⁻⁴M）不会改变乙酰胆碱的诱导作用。制剂储存70 - 90天后，乙酰胆碱的作用变为相反。该现象可通过微粒体在37℃“老化”3 - 6小时来模拟。
