Department of Cardiovasology, Department of Hypertension and Vascular Disease, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong, China.
Neurochem Res. 2011 Nov;36(11):1955-61. doi: 10.1007/s11064-011-0518-y. Epub 2011 Jun 4.
Nogo-A, a major myelin inhibitory protein, inhibits axon growth and synaptic function in the central nervous system. Glaucoma is a progressive neuropathy as a result of retinal ganglion cell (RGC) death. Synaptic degeneration is thought to be an early pathology of neurodegeneration in glaucoma and precedes RGC loss. Here experimental ocular hypertension model was induced in adult rats with laser coagulation of the episcleral and limbal veins. The expression of Nogo-A, myelin-associated glycoprotein (MAG) and oligodendrocyte myelin glycoprotein (OMgp) in the retina was investigated using immunohistochemistry and Western blotting. We found that Nogo-A was expressed in the RGCs and upregulated after the induction of ocular hypertension. OMgp was only expressed in the inner plexiform layer. There was no MAG expression in the retina. Our data provided, for the first time, the expression patterns of three myelin proteins in the adult retina and suggested an important role of Nogo-A in the RGC death and synaptic degeneration in glaucoma.
Nogo-A 是一种主要的髓鞘抑制蛋白，可抑制中枢神经系统中的轴突生长和突触功能。青光眼是一种进行性神经病变，是由于视网膜神经节细胞（RGC）死亡引起的。突触退化被认为是青光眼神经退行性变的早期病理学，并先于 RGC 丧失。在这里，通过对巩膜和角膜缘静脉进行激光凝固，在成年大鼠中诱导了实验性高眼压模型。使用免疫组织化学和 Western blot 研究了视网膜中 Nogo-A、髓鞘相关糖蛋白（MAG）和少突胶质细胞髓鞘糖蛋白（OMgp）的表达。我们发现 Nogo-A 在 RGC 中表达，并在眼压升高后上调。OMgp 仅在内丛状层表达。视网膜中没有 MAG 表达。我们的数据首次提供了三种髓鞘蛋白在成年视网膜中的表达模式，并表明 Nogo-A 在青光眼的 RGC 死亡和突触退化中起重要作用。
