Dely M, Zsoldos T, Puppi A, Tigyi A
Central Laboratory of Animal Research, University Medical School, Pécs, Hungary.
Chem Biol Interact. 1990;75(2):213-23. doi: 10.1016/0009-2797(90)90119-8.
Homogenates of heart, stomach and rectus abdominis muscles of the frog have shown different degrees of malondialdehyde (MDA) formation. MDA content was highest in heart, and lowest in stomach musculature. The resultant tissue redox-state potential (RSP) and redox potential (E'0) in homogenates determined potentiometrically also showed differences with opposite signs in relation to MDA levels. An electron acceptor, methylene blue (MB), decreased but an electron donor, ascorbate (Asc), increased the MDA level in each of the muscles. These effects were dependent upon the concentration of MB and Asc and proportional to the control MDA content in each muscle. Thus an inverse interdependence between MDA level and redox state existed even when a positive change in redox potentials was induced by MB, and also when a negative change was induced by Asc. Since there was a close negative correlation between the changes of MDA concentration and redox potential in the homogenates, it is strongly suggested that the changes of redox state in muscle are implicated in the processes leading to lipid peroxidation (LP).
青蛙心脏、胃和腹直肌的匀浆呈现出不同程度的丙二醛（MDA）生成。MDA含量在心脏中最高，在胃肌肉组织中最低。通过电位法测定的匀浆中最终的组织氧化还原状态电位（RSP）和氧化还原电位（E'0）也显示出与MDA水平相反的差异。电子受体亚甲蓝（MB）降低了，但电子供体抗坏血酸（Asc）增加了每块肌肉中的MDA水平。这些效应取决于MB和Asc的浓度，并且与每块肌肉中的对照MDA含量成正比。因此，即使当MB引起氧化还原电位的正向变化，以及Asc引起负向变化时，MDA水平与氧化还原状态之间也存在反向相互依存关系。由于匀浆中MDA浓度变化与氧化还原电位之间存在密切的负相关，强烈表明肌肉中氧化还原状态的变化与导致脂质过氧化（LP）的过程有关。
