Laboratoire de Réactivité de Surface, Université Pierre et Marie Curie -Paris 06, CNRS UMR 7197, 4 Place Jussieu, Case 178, F-75005 Paris, France.
ACS Appl Mater Interfaces. 2011 Jul;3(7):2637-42. doi: 10.1021/am200442r. Epub 2011 Jun 28.
We report the synthesis of diacid-terminated PEG-functionalized cubic TiO(2) nanocrystals by a simple one-step solvothermal method, and their further use to form nanostructured surfaces for protein immobilization. The relevance and major interest of the so-obtained nanocrystals are the presence of terminal carboxylic acid groups at their surface, as confirmed by infrared analyses, in addition to the surrounding PEG chains, essential to avoid non specific interactions. These functional chemical groups were used to (i) immobilize the synthesized nanocubes on a cysteamine-modified Au surface, and to (ii) attach proteins via a presumable covalent link. AFM images show that the shapes and the narrow size distribution of the nanocubes, observed by TEM, were preserved after their immobilization on the modified Au surface. Moreover, the efficiency and specificity of antigen recognition were demonstrated using spectroscopic analyses. Our successful approach provides a versatile and facile way to elaborate specific and sensitive nanostructured surfaces for biosensors.
我们通过一种简单的一步溶剂热法合成了端酸基官能化的立方 TiO(2) 纳米晶，并进一步将其用于形成用于蛋白质固定化的纳米结构表面。所得到的纳米晶的相关性和主要兴趣在于其表面存在末端羧酸基团，这一点通过红外分析得到了证实，此外还有周围的 PEG 链，这对于避免非特异性相互作用是必要的。这些功能化学基团被用于 (i) 将合成的纳米立方体制备在半胱胺修饰的 Au 表面上，以及 (ii) 通过假定的共价键连接附着蛋白质。AFM 图像表明，TEM 观察到的纳米立方体制备在修饰的 Au 表面上后，其形状和窄的尺寸分布得以保留。此外，使用光谱分析证明了抗原识别的效率和特异性。我们成功的方法为生物传感器提供了一种通用且简便的方法来制备特异性和敏感性的纳米结构表面。
