Prigent A F, Fonlupt P, Dubois M, Némoz G, Timouyasse L, Pacheco H, Pacheco Y, Biot N, Perrin-Fayolle M
Unité 205 INSERM, Institut National des Sciences appliquées de Lyon, France.
Eur J Clin Invest. 1990 Jun;20(3):323-9. doi: 10.1111/j.1365-2362.1990.tb01864.x.
Cyclic nucleotide phosphodiesterase and phospholipid N-methyl-transferase activities were simultaneously measured in purified polymorphonuclear cell-, mononuclear cell-, lymphocyte- and monocyte-homogenates from control subjects, from patients with atopic asthma and from patients with non-atopic asthma. Whereas cyclic AMP and cyclic GMP phosphodiesterase activities were found to be about 10-fold lower in polymorphonuclear than in mononuclear cells, phospholipid N-methyltransferase proved to be rather similar in each cell type from control donors. Cyclic AMP phosphodiesterase and phospholipid N-methyltransferase were significantly decreased in polymorphonuclear cells and monocytes from asthmatic patients compared with the control group while cyclic GMP phosphodiesterase was significantly impaired only in the monocyte subpopulation.
在来自健康对照者、特应性哮喘患者和非特应性哮喘患者的纯化多形核细胞、单核细胞、淋巴细胞和单核细胞匀浆中，同时测定了环核苷酸磷酸二酯酶和磷脂N-甲基转移酶的活性。结果发现，多形核细胞中环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）磷酸二酯酶的活性比单核细胞中的低约10倍，而来自对照供体的每种细胞类型中，磷脂N-甲基转移酶的活性相当相似。与对照组相比，哮喘患者的多形核细胞和单核细胞中环磷酸腺苷磷酸二酯酶和磷脂N-甲基转移酶显著降低，而环磷酸鸟苷磷酸二酯酶仅在单核细胞亚群中显著受损。
