Silva M A, Peixoto G C X, Sousa P C, Bezerra F S B, Bezerra A C D S, Silva A R
Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil.
Reprod Domest Anim. 2012 Feb;47(1):e4-6. doi: 10.1111/j.1439-0531.2011.01817.x. Epub 2011 Jun 3.
This study verifies the interactions between straw size and thawing rates and their impact on the epididymal sperm from this species. Caudae epididymidum from 10 agoutis were subjected to retrograde washing using a coconut water extender (ACP-109c(®) ). Epididymal sperm were evaluated and extended in ACP-109c(®) plus egg yolk (20%) and glycerol (6%). The samples were packaged in 0.25- or 0.50-ml straws, frozen in liquid nitrogen and thawed at 37°C/1 min or 70°C/8 s, followed by a re-evaluation. The use of 0.25-ml straws thawed at 37°C/1 min provided a value of 26.6% for sperm motility. No interactions between straw size and thawing rates were verified on agouti sperm (p > 0.05), but when 0.5-ml straws were thawed at 70°C/8 s, sperm vigour decreased significantly (p < 0.05). It is recommended that the agouti epididymal sperm cryopreserved in ACP-109c(®) extender should be packaged in 0.25- or 0.50-ml straws and thawed at 37°C/60 s.
本研究验证了麦管规格与解冻速率之间的相互作用及其对该物种附睾精子的影响。对10只刺豚鼠的附睾尾部进行逆行冲洗，使用椰子水稀释液（ACP - 109c(®)）。对附睾精子进行评估，并在ACP - 109c(®)加蛋黄（20%）和甘油（6%）中进行稀释。将样品包装在0.25毫升或0.50毫升的麦管中，在液氮中冷冻，然后在37°C/1分钟或70°C/8秒解冻，随后再次评估。使用在37°C/1分钟解冻的0.25毫升麦管时，精子活力值为26.6%。在刺豚鼠精子上未验证麦管规格和解冻速率之间的相互作用（p > 0.05），但当0.5毫升麦管在70°C/8秒解冻时，精子活力显著下降（p < 0.05）。建议将保存在ACP - 109c(®)稀释液中的刺豚鼠附睾精子包装在0.25毫升或0.50毫升的麦管中，并在37°C/60秒解冻。
