Laboratory of Animal Germplasm Conservation, LCGA, Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil.
Theriogenology. 2012 Aug;78(3):605-11. doi: 10.1016/j.theriogenology.2012.03.006. Epub 2012 Apr 24.
The objective was to determine the effectiveness of a powdered coconut water-based extender (ACP-116c), plus various concentrations of egg-yolk and glycerol, as an alternative for cryopreservation of collared peccary semen. Twelve ejaculates were obtained from captive adult males by electroejaculation, and evaluated for sperm motility, kinetic rating, viability, morphology, and functional membrane integrity. The ejaculates were apportioned into aliquots that were diluted in Tris plus 10% egg yolk and 3% glycerol, or in ACP-116c plus 10 or 20% egg yolk and 1.5 or 3% glycerol. Samples were frozen in liquid nitrogen and, after 1 mo, thawed at 37 °C for 1 min. After thawing, samples were evaluated as reported for fresh semen, and also for sperm membrane integrity (fluorescent probes) and kinematic parameters (computerized analysis). Results were presented as means ± SEM. Freezing and thawing decreased sperm characteristics relative to fresh semen. Overall, ACP-116c plus 20% egg yolk and 3% glycerol provided better (P < 0.05) sperm motility and kinetic rating (48 ± 6.1% and 2.8 ± 0.2, respectively) after thawing than Tris extender (30.4 ± 5.7% and 2.4 ± 0.2). However, there were no differences (P > 0.05) among treatments with regard to the other sperm characteristics. Based on computerized motion analysis, total (26.5 ± 5.9%) and progressive (8.1 ± 2.2%) motility were best preserved (P < 0.05) with the above-mentioned treatment. In conclusion, a coconut water-based extender, ACP-116c, plus 20% egg yolk and 3% glycerol, was effective for cryopreservation of semen from collared peccaries.
目的是确定粉状椰子水基稀释剂（ACP-116c）与不同浓度的蛋黄和甘油结合作为野猪精液冷冻保存的替代物的有效性。通过电刺激法从圈养成年雄性中获得 12 份精液，评估精子活力、运动评分、活力、形态和功能膜完整性。将精液分配到等份中，用 Tris 加 10%蛋黄和 3%甘油稀释，或用 ACP-116c 加 10%或 20%蛋黄和 1.5%或 3%甘油稀释。将样品在液氮中冷冻，1 个月后在 37°C 下解冻 1 分钟。解冻后，按照新鲜精液的评估方法评估样品，还评估精子膜完整性（荧光探针）和运动学参数（计算机分析）。结果以平均值±SEM 表示。冷冻和解冻会降低精子相对于新鲜精液的特性。总体而言，ACP-116c 加 20%蛋黄和 3%甘油在解冻后提供更好的精子活力和运动评分（分别为 48±6.1%和 2.8±0.2），优于 Tris 稀释剂（分别为 30.4±5.7%和 2.4±0.2）。然而，在其他精子特性方面，不同处理之间没有差异（P＞0.05）。基于计算机运动分析，总活力（26.5±5.9%）和前向运动活力（8.1±2.2%）保存最好（P＜0.05），采用上述处理。总之，基于椰子水的稀释剂 ACP-116c 加 20%蛋黄和 3%甘油对野猪精液的冷冻保存有效。
