Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland.
J Appl Microbiol. 2011 Aug;111(2):474-83. doi: 10.1111/j.1365-2672.2011.05032.x. Epub 2011 Jun 6.
The aims of this study were to identify antifungal lactic acid bacteria (LAB) and characterize their activity against the dermatophyte Trichophyton tonsurans.
A total of 165 different LAB were isolated and initially screened for anti-Penicillium expansum activity. Five strains, which exhibited strong inhibitory activity, were then tested against the dermatophyte T. tonsurans DSM12285, where they also caused inhibition as observed by large fungal clearing on agar surface. The strongest inhibition was seen with Lactobacillus reuteri R2. When freeze-dried cell-free supernatant powder from this strain was incorporated in culture medium at concentrations >1%, growth of fungal colony was inhibited. Conidia germination was also inhibited under these conditions as determined by microscopy. The anti-T. tonsurans activity of Lact. reuteri R2 was not affected neither by heat treatment nor by proteolytic treatment using pronase E and proteinase K, indicating that the responsible agent(s) were nonproteinaceous in nature.
Lactobacillus reuteri R2 was identified as having strong inhibitory activity against the dermatophyte T. tonsurans DSMZ12285.
LAB are naturally associated with many foods and are well recognized for their biopreservative properties. The use of these and/or their products may well provide alternative safe approaches for the inhibition of dermatophytic fungi.
本研究旨在鉴定抗真菌乳酸菌（LAB），并研究其对亲动物性皮肤癣菌须癣毛癣菌的活性。
共分离出 165 株不同的 LAB，最初对其抗扩展青霉活性进行筛选。随后，对表现出强烈抑制活性的 5 株菌进行亲动物性皮肤癣菌须癣毛癣菌 DSM12285 的测试，在琼脂表面观察到大量真菌清除，表明这些菌株也具有抑制作用。其中，罗伊氏乳杆菌 R2 的抑制作用最强。当在培养基中加入该菌株的冻干无细胞上清粉，浓度>1%时，真菌菌落的生长受到抑制。在这些条件下，通过显微镜观察到孢子萌发也受到抑制。罗伊氏乳杆菌 R2 的抗须癣毛癣菌活性不受热处理和用蛋白酶 E 和蛋白激酶进行的蛋白水解处理的影响，表明其作用因子是非蛋白质性质的。
罗伊氏乳杆菌 R2 对亲动物性皮肤癣菌须癣毛癣菌 DSMZ12285 具有强烈的抑制活性。
LAB 与许多食品密切相关，其生物保鲜特性已得到广泛认可。这些细菌及其产品的使用可能为抑制皮肤真菌提供安全替代方法。
