Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India.
Department of Microbiology, Defence Food Research Laboratory, Siddarthnagar, Mysuru, Karnataka, India.
Probiotics Antimicrob Proteins. 2019 Mar;11(1):55-64. doi: 10.1007/s12602-017-9338-y.
The study aims to evaluate the cell-free supernatant (CFS) from Lactobacillus plantarum strain MYS44 against the growth and aflatoxin production by Aspergillus parasiticus MTCC 411. Standard in vitro techniques revealed the potential antifungal activity of CFS of LpMYS44. In poison food technique, it was observed that 6% CFS of LpMYS44 retarded maximum growth. The inhibition of A. parasiticus on peanuts confirmed the ability of CFS of LpMYS44 for biopreservation. Further, CFS of LpMYS44 was purified by chromatography and analyzed by GC-MS. The major antifungal compounds were oleic acid, octanoic acid, butanamide, and decanoic acid derivatives. Twofold concentrated 80 μL of CFS was found to be minimum inhibitory concentration (MIC) of CFS of LpMYS44. CFS of LpMYS44 suppressed the germination and growth of the spores of A. parasiticus. Microscopic observation showed that CFS of LpMYS44 severely affected the hyphal wall of A. parasiticus by the leakage of cytoplasmic content leading to complete destruction. Acidic condition is favorable for CFS of LpMYS44 activity. In poultry feed sample, CFS of LpMYS44 reduced the aflatoxin B content by 34.2%, reflecting its potentiality to use as detoxification agent. The multiple antifungal components in CFS of LpMYS44 exhibited antifungal properties against aflatoxigenic A. parasiticus resulted in causing overall morphological changes. Furthermore, we also observed the biopreservative ability of CFS of LpMYS44 against A. parasiticus and AFB reduction in for poultry feed. This study makes a contribution to using CFS of LpMYS44 and their applications in food and feed as pretreatment against aflatoxigenic A. parasiticus to reduce or eliminate AFB and maybe other aflatoxins, produced by other Aspergillus spp.
本研究旨在评估植物乳杆菌 MYS44 的无细胞上清液（CFS）对寄生曲霉 MTCC 411 的生长和黄曲霉毒素产生的抑制作用。标准的体外技术显示了 LpMYS44 的 CFS 具有潜在的抗真菌活性。在毒饵技术中，观察到 6%的 LpMYS44 的 CFS 延缓了最大生长。CFS 的花生抑制作用证实了 LpMYS44 的生物保鲜能力。此外，通过色谱法对 LpMYS44 的 CFS 进行了纯化，并通过 GC-MS 进行了分析。主要的抗真菌化合物是油酸、辛酸、丁酰胺和癸酸衍生物。发现两倍浓缩的 80μL 的 CFS 是 LpMYS44 的 CFS 的最小抑菌浓度（MIC）。LpMYS44 的 CFS 抑制了寄生曲霉孢子的萌发和生长。显微镜观察显示，LpMYS44 的 CFS 严重影响了寄生曲霉的菌丝壁，导致细胞质内容物泄漏，从而造成完全破坏。酸性条件有利于 LpMYS44 的 CFS 活性。在禽饲料样品中，LpMYS44 的 CFS 将黄曲霉毒素 B 的含量降低了 34.2%，反映了其作为解毒剂的潜力。LpMYS44 的 CFS 中的多种抗真菌成分表现出对产黄曲霉毒素的寄生曲霉的抗真菌特性，导致其整体形态发生变化。此外，我们还观察到 LpMYS44 的 CFS 对寄生曲霉和禽用饲料中 AFB 减少的生物保鲜能力。本研究为使用 LpMYS44 的 CFS 及其在食品和饲料中的应用提供了依据，可作为预处理方法，以抑制产黄曲霉毒素的寄生曲霉，从而降低或消除 AFB 以及可能由其他曲霉属产生的其他黄曲霉毒素。
