Kim Chul-Hong, Lillehoj Hyun S, Hong Yeong-Ho, Keeler Calvin L, Lillehoj Erik P
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, USDA, Beltsville, MD 20705, USA.
BMC Proc. 2011 Jun 3;5 Suppl 4(Suppl 4):S12. doi: 10.1186/1753-6561-5-S4-S12.
Characterization of host transcriptional responses during coccidia infections can provide new clues for the development of alternative disease control strategies against these complex protozoan pathogens.
In the current study, we compared chicken duodenal transcriptome profiles following primary and secondary infections with Eimeria acervulina using a 9.6K avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA).
Gene Ontology analysis showed that primary infection significantly modulated the levels of mRNAs for genes involved in the metabolism of lipids and carbohydrates as well as those for innate immune-related genes. By contrast, secondary infection increased the levels of transcripts encoded by genes related to humoral immunity and reduced the levels of transcripts for the innate immune-related genes. The observed modulation in transcript levels for gene related to energy metabolism and immunity occurred concurrent with the clinical signs of coccidiosis.
Our results suggest that altered expression of a specific set of host genes induced by Eimeria infection may be responsible, in part, for the observed reduction in body weight gain and inflammatory gut damage that characterizes avian coccidiosis.
球虫感染期间宿主转录反应的特征可为开发针对这些复杂原生动物病原体的替代疾病控制策略提供新线索。
在本研究中，我们使用9.6K禽类肠道上皮内淋巴细胞cDNA微阵列（AVIELA）比较了初次和二次感染堆型艾美耳球虫后鸡十二指肠的转录组谱。
基因本体分析表明，初次感染显著调节了参与脂质和碳水化合物代谢的基因以及先天免疫相关基因的mRNA水平。相比之下，二次感染增加了体液免疫相关基因编码的转录本水平，并降低了先天免疫相关基因的转录本水平。观察到的与能量代谢和免疫相关基因的转录水平调节与球虫病的临床症状同时出现。
我们的结果表明，艾美耳球虫感染诱导的一组特定宿主基因表达的改变可能部分导致了观察到的体重增加减少和肠道炎症损伤，这是禽类球虫病的特征。
