Department of Chemistry and Chemical Engineering, East China Jiao Tong University, Nanchang, Jiangxi 330013, PR China.
Talanta. 2011 Jul 15;85(1):43-8. doi: 10.1016/j.talanta.2011.02.048. Epub 2011 Mar 4.
In this paper, a polydopamine (PDA) film is electropolymerized on the surface of bilayer lipid membrane (BLM) which is immobilized with horseradish peroxidase (HRP). The coverage of the PDA film on HRP/BLM electrode is monitored by electrochemical impedance spectroscopy (EIS). The electrocatalytic reduction of H(2)O(2) at the PDA/HRP/BLM electrode is studied by means of cyclic voltammetry (CV). The biosensor has a fast response to H(2)O(2) of less than 5s and an excellent linear relationship is obtained in the concentration range from 2.5×10(-7) to 3.1×10(-3) molL(-1), with a detection limit of 1.0×10(-7) molL(-1) (S/N=3). The response current of BLM/HRP/PDA biosensor retains 84% of its original response after being stored in 0.1 molL(-1) pH 7.0 PBS at 4°C for 3 weeks. The selectivity, repeatability, and storage stability of PDA/HRP/BLM biosensor are greatly enhanced by the coverage of polydopamine film on BLM.
本文在固定有辣根过氧化物酶（HRP）的双层脂质膜（BLM）表面电聚合聚多巴胺（PDA）薄膜。通过电化学阻抗谱（EIS）监测 PDA 薄膜在 HRP/BLM 电极上的覆盖度。通过循环伏安法（CV）研究 PDA/HRP/BLM 电极上 H₂O₂的电催化还原。该生物传感器对 H₂O₂的快速响应时间小于 5s，在 2.5×10⁻⁷~3.1×10⁻³molL⁻¹浓度范围内具有良好的线性关系，检测限为 1.0×10⁻⁷molL⁻¹（S/N=3）。将 BLM/HRP/PDA 生物传感器储存在 4°C 下 0.1 molL⁻¹ pH 7.0 PBS 中 3 周后，其响应电流保持原始响应的 84%。聚多巴胺薄膜对 BLM 的覆盖大大提高了 PDA/HRP/BLM 生物传感器的选择性、重现性和储存稳定性。
