双层脂质膜生物传感器，稳定性增强，用于电流测定法测定过氧化氢。
Bilayer lipid membrane biosensor with enhanced stability for amperometric determination of hydrogen peroxide.
机构信息
Department of Chemistry and Chemical Engineering, East China Jiao Tong University, Nanchang, Jiangxi 330013, PR China.
出版信息
Talanta. 2011 Jul 15;85(1):43-8. doi: 10.1016/j.talanta.2011.02.048. Epub 2011 Mar 4.
In this paper, a polydopamine (PDA) film is electropolymerized on the surface of bilayer lipid membrane (BLM) which is immobilized with horseradish peroxidase (HRP). The coverage of the PDA film on HRP/BLM electrode is monitored by electrochemical impedance spectroscopy (EIS). The electrocatalytic reduction of H(2)O(2) at the PDA/HRP/BLM electrode is studied by means of cyclic voltammetry (CV). The biosensor has a fast response to H(2)O(2) of less than 5s and an excellent linear relationship is obtained in the concentration range from 2.5×10(-7) to 3.1×10(-3) molL(-1), with a detection limit of 1.0×10(-7) molL(-1) (S/N=3). The response current of BLM/HRP/PDA biosensor retains 84% of its original response after being stored in 0.1 molL(-1) pH 7.0 PBS at 4°C for 3 weeks. The selectivity, repeatability, and storage stability of PDA/HRP/BLM biosensor are greatly enhanced by the coverage of polydopamine film on BLM.
本文在固定有辣根过氧化物酶（HRP）的双层脂质膜（BLM）表面电聚合聚多巴胺（PDA）薄膜。通过电化学阻抗谱（EIS）监测 PDA 薄膜在 HRP/BLM 电极上的覆盖度。通过循环伏安法（CV）研究 PDA/HRP/BLM 电极上 H₂O₂的电催化还原。该生物传感器对 H₂O₂的快速响应时间小于 5s，在 2.5×10⁻⁷~3.1×10⁻³molL⁻¹浓度范围内具有良好的线性关系，检测限为 1.0×10⁻⁷molL⁻¹（S/N=3）。将 BLM/HRP/PDA 生物传感器储存在 4°C 下 0.1 molL⁻¹ pH 7.0 PBS 中 3 周后，其响应电流保持原始响应的 84%。聚多巴胺薄膜对 BLM 的覆盖大大提高了 PDA/HRP/BLM 生物传感器的选择性、重现性和储存稳定性。
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