Department de Medicina Preventiva, Facultat de Farmàcia, Universitat de Valencia, Av. Vicent Andrès Estellès s/n. 46100 Burjassot, Spain.
Talanta. 2011 Jul 15;85(1):206-15. doi: 10.1016/j.talanta.2011.03.046. Epub 2011 Mar 30.
An existing matrix solid-phase dispersion (MSPD) method for aflatoxins (AFs) and ochratoxin A (OTA) extraction was extended by further 14 mycotoxins. After it careful optimization, this method was applied to determine the occurrence of these mycotoxins on commercial flour samples (with different cereals composition) collected from local markets. In a total of 49 samples investigated, 9 mycotoxins were identified. Nivalenol (NIV) and Beauvericin (BEA) were the mycotoxins found most frequently. The samples that presented major contamination were wheat flours and bakery preparations. Despite of the great number of positives finding, only one wheat flour sample exceeded the maximum limits (ML) for OTA established by the European Union (EU). However, it would be interesting to calculate the total ingest of these mycotoxins along the years.
现有的基质固相分散（MSPD）方法可用于提取黄曲霉毒素（AFs）和赭曲霉毒素 A（OTA），本研究将其进一步扩展，可用于提取 14 种其他真菌毒素。在经过仔细优化后，该方法被应用于测定从当地市场采集的商业面粉样品（不同谷物成分）中这些真菌毒素的存在情况。在所研究的 49 个样本中，共鉴定出 9 种真菌毒素。雪腐镰刀菌烯醇（NIV）和布氏菌素（BEA）是最常被发现的真菌毒素。受污染最严重的是小麦粉和烘焙制品。尽管检出了大量阳性样本，但只有一个小麦粉样本超过了欧盟（EU）规定的 OTA 最大限量（ML）。然而，计算这些真菌毒素多年来的总摄入量将是很有趣的。
