State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Talanta. 2011 Jul 15;85(1):325-32. doi: 10.1016/j.talanta.2011.03.067. Epub 2011 Apr 5.
A rapid non-separative spectrofluorometric method based on the second-order calibration of excitation-emission matrix (EEM) fluorescence was proposed for the determination of napropamide (NAP) in soil, river sediment, and wastewater as well as river water samples. With 0.10 mol L(-1) sodium citrate-hydrochloric acid (HCl) buffer solution of pH 2.2, the system of NAP has a large increase in fluorescence intensity. To handle the intrinsic fluorescence interferences of environmental samples, the alternating penalty trilinear decomposition (APTLD) algorithm as an efficient second-order calibration method was employed. Satisfactory results have been achieved for NAP in complex environmental samples. The limit of detection obtained for NAP in soil, river sediment, wastewater and river water samples were 0.80, 0.24, 0.12, 0.071 ng mL(-1), respectively. Furthermore, in order to fully investigate the performance of second-order calibration method, we test the second-order calibration method using different calibration approaches including the single matrix model, the intra-day various matrices model and the global model based on the APTLD algorithm with nature environmental datasets. The results showed the second-order calibration methods also enable one or more analyte(s) of interest to be determined simultaneously in the samples with various types of matrices. The maintenance of second-order advantage has been demonstrated in simultaneous determinations of the analyte of interests in the environmental samples of various matrices.
一种基于激发-发射矩阵（EEM）荧光二阶校正的快速非分离分光荧光法被提出，用于测定土壤、河沉积物、废水和河水样品中的萘丙胺（NAP）。在 pH 2.2 的 0.10 mol L(-1) 柠檬酸钠-盐酸（HCl）缓冲溶液中，NAP 体系的荧光强度有很大的增加。为了处理环境样品中固有的荧光干扰，采用交替惩罚三线性分解（APTLD）算法作为有效的二阶校正方法。该方法在复杂环境样品中对 NAP 取得了令人满意的结果。在土壤、河沉积物、废水和河水中，NAP 的检出限分别为 0.80、0.24、0.12 和 0.071 ng mL(-1)。此外，为了充分研究二阶校正方法的性能，我们使用不同的校正方法，包括单矩阵模型、日内各种基质模型和基于 APTLD 算法的全局模型，对二阶校正方法进行了测试，这些方法都是基于自然环境数据集。结果表明，二阶校正方法也能够同时在多种基质的样品中同时测定一种或多种感兴趣的分析物。在各种基质的环境样品中对感兴趣的分析物的同时测定中，二阶优势得以保持。
