DVGW Water Technology Center Karlsruhe, Branch Dresden, Wasserwerkstraße 2, 01326 Dresden, Germany.
Talanta. 2011 Jul 15;85(1):650-6. doi: 10.1016/j.talanta.2011.04.045. Epub 2011 Apr 22.
Fluorescence excitation-emission-matrices (EEM) are a useful tool for water quality monitoring. Recent publications show the potential of the method for real time drinking water control. However, in fluorescence measurements there is still a need for standardization to make data interpretation comparable. In this work a standardization procedure based on excitation and emission correction as well as normalization and optional inner filter effect correction is presented. By measurements of humic acid and tryptophan standards with two different spectrometers (LS 50 and LS 55 by PerkinElmer) the procedure application leads to comparable fluorescence intensities with relative standard deviations (median) of 6.6-8.4% and 10.6-12.0%, respectively. These small differences are not avoidable even if all possible correction methods are implemented and constant measurement conditions are given. The used BAM kit for emission correction induced good agreement in peak shape not only for single wavelengths but also for the whole EEM. As a consequence it is necessary to use identical equipment and identical experimental conditions in order to apply this method in fields of water quality control if small changes of fluorescence intensities are relevant for data assessment.
荧光激发-发射矩阵（EEM）是水质监测的一种有用工具。最近的出版物表明，该方法具有实时饮用水控制的潜力。然而，在荧光测量中，仍然需要标准化，以使数据解释具有可比性。在这项工作中，提出了一种基于激发和发射校正以及归一化和可选内滤光效应校正的标准化程序。通过用两台不同的光谱仪（PerkinElmer 的 LS 50 和 LS 55）测量腐殖酸和色氨酸标准品，该程序的应用导致荧光强度具有可比性，相对标准偏差（中位数）分别为 6.6-8.4%和 10.6-12.0%。即使实施了所有可能的校正方法并给出了恒定的测量条件，这些小的差异也是不可避免的。所使用的 BAM 试剂盒进行发射校正不仅在单个波长而且在整个 EEM 中都产生了良好的峰形一致性。因此，如果荧光强度的微小变化对数据评估很重要，那么为了在水质控制领域应用该方法，有必要使用相同的设备和相同的实验条件。
