基于金纳米粒子猝灭罗丹明 B 荧光的无标记 DNA 检测。
Label free DNA detection based on gold nanoparticles quenching fluorescence of Rhodamine B.
机构信息
School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, PR China.
出版信息
Talanta. 2011 Jul 15;85(1):725-9. doi: 10.1016/j.talanta.2011.04.057. Epub 2011 Apr 29.
A novel and sensitive label free DNA detection method using gold nanoparticles (GNPs) and Rhodamine B (RB) has been developed. The assay is based on the following two properties. One is the different adsorption properties of single-stranded and double-stranded DNA on GNPs in colloidal solution. The other is the different quenching ability of aggregated GNPs and dispersed GNPs on RB. Un-aggregated GNPs could effectively quench the fluorescence of RB. However, the quenching ability greatly decreases after GNPs aggregated. The hybridization of probe DNA and target DNA is monitored by the fluorescence detection after the RB is added to the solution. Under the optimal experimental conditions, the detection limit of this assay is 2.9×10(-13) mol L(-1).
一种使用金纳米粒子（GNPs）和罗丹明 B（RB）的新型灵敏无标记 DNA 检测方法已经开发出来。该测定法基于以下两个特性。一个是在胶体溶液中单链和双链 DNA 在 GNPs 上的不同吸附特性。另一个是聚集的 GNPs 和分散的 GNPs 对 RB 的不同猝灭能力。未聚集的 GNPs 可以有效地猝灭 RB 的荧光。然而，GNPs 聚集后猝灭能力大大降低。在向溶液中加入 RB 后，通过荧光检测来监测探针 DNA 和靶 DNA 的杂交。在最佳实验条件下，该测定法的检测限为 2.9×10(-13)mol L(-1)。
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