Farajzadeh Nazli, Ranjbar Nader Nasrin
Department of Chemistry, Faculty of Science and Technology, İstanbul Technical University,İstanbul Turkey.
Turk J Chem. 2020 Jun 1;44(3):656-669. doi: 10.3906/kim-1912-6. eCollection 2020.
An inexpensive, simple, highly sensitive, and rapid fluorimetric method was developed for the analysis of pseudoephedrine hydrochloride at trace levels. The method is based on the recovery of fluorescence of Rh6G dye due to the interaction of pseudoephedrine hydrochloride with Rh6G-Au NPs complex, which results in the release of Rh6G from the complex and halting fluorescence resonance energy transfer between Rh6G and Au NPs. The intensity of fluorescence was directly proportional to the concentration of the analyte, which was used for its determination. Experimental factors were optimized by response-surface methodology. Under optimum conditions, the calibration curve was linear over the range of 15-150 ng mL and the limit of detection (LOD) was 10 ng mL. Percent relative standard deviation (n= 5) for determination of 50 ng mL pseudoephedrine hydrochloride was 3.74%. The method was successfully used for determining the analyte in human blood serum and in pharmaceutical formulations. The possible mechanistic description of the analytical reaction was proposed on the basis of TEM, FT-IR, and fluorescence spectra analysis.
开发了一种用于分析痕量盐酸伪麻黄碱的廉价、简单、高灵敏度且快速的荧光方法。该方法基于盐酸伪麻黄碱与罗丹明6G-金纳米粒子（Rh6G-Au NPs）复合物相互作用导致罗丹明6G染料荧光恢复，这使得Rh6G从复合物中释放出来，并终止Rh6G与Au NPs之间的荧光共振能量转移。荧光强度与分析物浓度成正比，用于其测定。通过响应面法对实验因素进行了优化。在最佳条件下，校准曲线在15 - 150 ng/mL范围内呈线性，检测限（LOD）为10 ng/mL。测定50 ng/mL盐酸伪麻黄碱时的相对标准偏差百分比（n = 5）为3.74%。该方法成功用于测定人血清和药物制剂中的分析物。基于透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）和荧光光谱分析，对分析反应的可能机理进行了描述。
