Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science, Brusselsesteenweg 370, 9090 Melle, Belgium.
Food Microbiol. 2011 Sep;28(6):1162-9. doi: 10.1016/j.fm.2011.03.009. Epub 2011 Apr 7.
This study points out the limitations of several general growth media frequently used in seafood research by a systematic identification of the microorganisms on fish samples during ice storage unable to grow on those media. Aerobic psychrotrophic count (APC), replication on various general media and total cultivable microbial community denaturing gradient gel electrophoresis (DGGE) analysis revealed that many potential spoilage microorganisms were overlooked. Those microorganisms overlooked by using only one single growth medium were identified by partial 16S rRNA gene and gyrB gene sequencing. Members of the genera Shewanella, Vibrio, Aliivibrio, Photobacterium, Pseudoalteromonas and Psychrobacter, including Photobacterium phosphoreum, Shewanella baltica and Pseudomonas fluorescens are unable to grow on PCA. APC analysis also confirmed that on plate count agar (PCA) the enumeration of the microbiota was underestimated. Although Long and Hammer agar (LH) and marine agar (MA) obtained the best quantitative (APC analysis) and qualitative (replication and DGGE analyses) results for fish quality analysis, analysts have to keep in mind that some species were also unable to grow on those media, such as Pseudomonas fragi and Acinetobacter sp.
本研究通过系统鉴定冰藏过程中无法在常用海鲜研究培养基上生长的鱼类样品中的微生物，指出了几种常用通用培养基的局限性。好氧冷适应菌（APC）计数、在各种普通培养基上的复制以及总可培养微生物群落变性梯度凝胶电泳（DGGE）分析表明，许多潜在的腐败微生物被忽视了。仅使用一种单一生长培养基会遗漏那些微生物，通过部分 16S rRNA 基因和 gyrB 基因测序进行鉴定。希瓦氏菌属、弧菌属、Aliivibrio 属、发光杆菌属、假交替单胞菌属和 Psychrobacter 属的成员，包括荧光假单胞菌、北海希瓦氏菌和荧光假单胞菌，无法在 PCA 上生长。APC 分析还证实，在平板计数琼脂（PCA）上，微生物菌群的计数被低估了。尽管 Long 和 Hammer 琼脂（LH）和海洋琼脂（MA）在鱼类质量分析中获得了最佳的定量（APC 分析）和定性（复制和 DGGE 分析）结果，但分析人员必须记住，有些物种也无法在这些培养基上生长，例如脆弱假单胞菌和不动杆菌属。
