Cai Wen-Qiang, Jiang Cai-Yan, Shang Shan, Wang Shu-Chen, Zhu Kai-Yue, Dong Xiu-Ping, Zhou Da-Yong, Jiang Peng-Fei
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
National Engineering Research Center of Seafood, Dalian 116034, China.
Food Chem X. 2023 May 29;18:100727. doi: 10.1016/j.fochx.2023.100727. eCollection 2023 Jun 30.
Sea urchin gonads have high nutritional value and degenerate rapidly during storage. Previous assessment of the freshness of sea urchin gonads was based on experience without valid biochemical indicators. Thus, the current study is to find biochemical indicators representing the freshness of sea urchin gonads. Results showed that the dominant genera of sea urchin gonads were changed from , , and to , , and . The differential metabolites of sea urchin gonads were mainly produced through amino acids metabolism. Among them, GC-TOF-MS based differential metabolites had the greatest enrichment in the valine, leucine and isoleucine biosynthesis pathway, while LC-MS based differential metabolites had the greatest enrichment in the alanine, aspartate and glutamate metabolism pathway. The growth of dominant genus () had a great influence on the production of differential metabolites. These results will provide valuable information for accurately judging the freshness and shelf life of sea urchin gonads.
海胆性腺具有较高的营养价值，但在储存过程中会迅速退化。以往对海胆性腺新鲜度的评估基于经验，缺乏有效的生化指标。因此，本研究旨在寻找代表海胆性腺新鲜度的生化指标。结果表明，海胆性腺的优势菌属从 、 和 变为 、 和 。海胆性腺的差异代谢物主要通过氨基酸代谢产生。其中，基于气相色谱-飞行时间质谱的差异代谢物在缬氨酸、亮氨酸和异亮氨酸生物合成途径中富集程度最高，而基于液相色谱-质谱的差异代谢物在丙氨酸、天冬氨酸和谷氨酸代谢途径中富集程度最高。优势菌属（）的生长对差异代谢物的产生有很大影响。这些结果将为准确判断海胆性腺的新鲜度和保质期提供有价值的信息。
