Microbial Adhesion Group, DTU Food, Technical University of Denmark, Lyngby, Denmark.
Int J Med Microbiol. 2011 Nov;301(7):570-6. doi: 10.1016/j.ijmm.2011.04.018. Epub 2011 Jun 6.
Biofilm formation is involved in the majority of bacterial infections. Comparing six Escherichia coli and Klebsiella pneumoniae isolates revealed significant differences in biofilm formation depending on the growth medium. Fimbriae are known to be involved in biofilm formation, and type 1, F1C and P fimbriae were seen to influence biofilm formation significantly different depending on strain background, growth media and aeration as well as surface material. Altogether, this report clearly demonstrates that biofilm formation of a given strain is highly dependent on experimental design and that specific mechanisms involved in biofilm formation such as fimbrial expression only play a role under certain environmental conditions. This study underscores the importance of careful selection of experimental conditions when investigating bacterial biofilm formation and to take great precaution/care when comparing results from different biofilm studies.
生物膜的形成与大多数细菌感染有关。比较六种大肠杆菌和肺炎克雷伯菌的分离株发现，生物膜的形成取决于生长培养基，存在显著差异。菌毛被认为参与生物膜的形成，1 型、F1C 和 P 菌毛的表达根据菌株背景、生长培养基、通气和表面材料的不同，对生物膜的形成有显著影响。总的来说，本报告清楚地表明，给定菌株的生物膜形成高度依赖于实验设计，而参与生物膜形成的特定机制，如菌毛表达，仅在某些环境条件下起作用。本研究强调了在研究细菌生物膜形成时，仔细选择实验条件的重要性，并在比较来自不同生物膜研究的结果时，要格外小心谨慎。
